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ACY3 Antibody (clone 2G2) LS‑C172578
ACY3 antibody LS-C172578 is an unconjugated mouse monoclonal antibody to human ACY3. Validated for Flow, IHC and WB.
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Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Monkey, Dog
Applications:
IHC, IHC - Paraffin, Western blot, Flow Cytometry
Species:
Human, Monkey
Applications:
IHC, IHC - Paraffin, Immunofluorescence, Western blot, Flow Cytometry
Product Description
ACY3 antibody LS-C172578 is an unconjugated mouse monoclonal antibody to human ACY3. Validated for Flow, IHC and WB.
About ACY3
Plays an important role in deacetylating mercapturic acids in kidney proximal tubules. Also acts on N-acetyl-aromatic amino acids.
AA894718
ACY3 Antibody, Acylase III Antibody, ACY-3 Antibody, Aminoacylase-3 Antibody, Aspartoacylase-2 Antibody, HCBP1 Antibody, HCV core-binding protein 1 Antibody, ASPA2 Antibody
Specifications
Target
Human ACY3
Host
Mouse
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG1
Monoclonal
[2G2]
Conjugations
Unconjugated
Purification
Protein A/G purified
Modifications
Unmodified
Applications
- IHC
- IHC - Paraffin (1:150)
- Western blot (1:200 - 1:500)
- Flow Cytometry (1:100)
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Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Immunogen
ACY3 antibody was raised against full length human recombinant protein of human ACY3 (NP_542389) produced in HEK293T cell.
Specificity
Human ACY3
Presentation
PBS, pH 7.3, 1% BSA, 50% glycerol, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
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Customer Reviews (0)
Images
Immunohistochemistry
IHC of paraffin-embedded Human Kidney tissue using anti-ACY3 mouse monoclonal antibody.
Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ACY3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY3.
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY3 antibody, and then analyzed by flow cytometry.
Immunohistochemistry
IHC of paraffin-embedded Human Kidney tissue using anti-ACY3 mouse monoclonal antibody.
Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ACY3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY3.
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY3 antibody, and then analyzed by flow cytometry.
Immunohistochemistry
IHC of paraffin-embedded Human Kidney tissue using anti-ACY3 mouse monoclonal antibody.
Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ACY3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY3.
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY3 antibody, and then analyzed by flow cytometry.
Immunohistochemistry
IHC of paraffin-embedded Human Kidney tissue using anti-ACY3 mouse monoclonal antibody.
Western blot
Western blot of extracts (35 ug) from 9 different cell lines by using anti-ACY3 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
Western blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY ACY3 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-ACY3.
Flow Cytometry
HEK293T cells transfected with either overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-ACY3 antibody, and then analyzed by flow cytometry.
Requested From:
Date Requested: 6/23/2018
Date Requested: 6/23/2018
