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Anti-ACTN2 Antibody IHC-plus™ LS-B4217

Note: This antibody replaces LS-C109365

Ordering

Wt. Vol. Conc. Price
- 50 µl 0.53 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACTN2 Antibodies

Anti-ACTN2 Antibody (C-Terminus) LS-C40329
Rabbit Polyclonal (IgG) to Human ACTN2
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Guinea pig, Horse, Pig, Rabbit, Xenopus, Zebrafish
IHC - Paraffin, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ACTN2 Antibody IHC-plus™ LS-B4217
Rabbit Polyclonal to Human ACTN2
Human, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image
Anti-ACTN2 Antibody IHC-plus™ LS-B4852
Rabbit Polyclonal to Human ACTN2
Human
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal to Human ACTN2
Human, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot
Unconjugated

Details

Human ACTN2
Rabbit
Human, Mouse (tested or 100% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • IHC - Paraffin (10 µg/ml)
  • ICC
  • Immunofluorescence (1:100 - 1:200)
  • Western blot (1:500 - 1:3000)

Specificity and Use

ACTN2 antibody was raised against recombinant protein fragment containing a sequence corresponding to a region within amino acids 11 and 347 of alpha Actinin 2.
Human and mouse alpha Actinin 2. Predicted cross-reactivity based on amino acid sequence homology: rat (91%), bovine (99%), zebrafish (92%).

Packaging

0.1 M Tris-glycine, pH 7, 20% Glycerol, 0.01% Thimerosal
Aliquot and store at -20°C. Minimize freezing and thawing.
For research use only.

About ACTN2

P35609 NM_001103 NP_001094.1

ACTN2 Antibody, Actinin, alpha 2 Antibody, Alpha-actinin skeletal muscle Antibody, CMD1AA Antibody, F-actin cross-linking protein Antibody, Alpha-actinin-2 Antibody

Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane.

Immunohistochemistry

Immunohistochemistry
Anti-ACTN2 antibody IHC of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4217 concentration 10 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-ACTN2 antibody IHC of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B4217 concentration 10 ug/ml.

Immunofluorescence

Immunofluorescence
Immunofluorescence of methanol-fixed HeLa, using alpha Actinin 2 antibody at 1:200 dilution.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: NIH-3T3. 7.5% SDS PAGE. ACTN2 antibody diluted at 1:1000.

Western blot

Western blot
Sample(30 ug whole cell lysate). A: A431. B: H1299. C: HeLa S3. D: Hep G2. 7.5% SDS PAGE. ACTN2 antibody diluted at 1:1000.
 

Requested From: United States
Date Requested: 12/9/2016

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