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Anti-ACTC1 / Alpha Cardiac Actin Antibody (aa99-354) IHC-plus™ LS-B10755

Note: This antibody replaces LS-C185441

Ordering

Wt. Vol. Conc. Price
- 100 µl 1 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
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Rabbit Polyclonal to Human ACTC1 / Alpha Cardiac Actin
Human, Rat, Bovine, Pig, Chicken
IHC - Paraffin, Western blot
Unconjugated

Details

Human ACTC1 / Alpha Cardiac Actin
Rabbit
Human, Rat, Bovine, Pig, Chicken (tested or 100% immunogen sequence identity)
Polyclonal
Immunoaffinity purified

Applications

IHC - Paraffin (7.5 µg/ml), Western blot (1:500 - 1:3000)

Specificity and Use

ACTC1 / Alpha Cardiac Actin antibody was raised against recombinant protein fragment contain a sequence corresponding to a region within amino acids 99 and 354 (P68032) of alpha Actin (cardiac muscle). Percent identity by BLAST analysis: Human, Chicken, Pig, Rat, Bovine (100%); Xenopus (99%).
aa99-354
Human ACTC1 / Alpha-Cardiac Actin
IHC-paraffin: Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Packaging

PBS, pH 7, 1% BSA, 20% Glycerol, 0.01% Thimerosal
Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
For research use only.

About ACTC1 / Alpha Cardiac Actin

P68032 NM_005159 NP_005150.1

ACTC1 Antibody, Actin, alpha cardiac muscle 1 Antibody, Actin, alpha, cardiac muscle Antibody, Actin, alpha, cardiac muscle 1 Antibody, Alpha-cardiac actin Antibody, CMH11 Antibody, CMD1R Antibody, ACTC Antibody, LVNC4 Antibody, ASD5 Antibody

Actins are highly conserved proteins that are involved in various types of cell motility. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to four others. The protein encoded by this gene belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus.

Immunohistochemistry

Immunohistochemistry
Anti-ACTC1 / Alpha Cardiac Actin antibody IHC staining of human heart. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B10755 concentration 7.5 ug/ml.

Immunohistochemistry

Immunohistochemistry
Anti-ACTC1 / Alpha Cardiac Actin antibody IHC staining of human skeletal muscle. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: HeLa S3, B: MOLT4. 12% SDS PAGE. ACTC1 / Alpha-Cardiac Actin antibody diluted at 1:500

Western blot

Western blot
Sample (30 ug of whole cell lysate). A: NIH-3T3. 10% SDS PAGE. ACTC1 / Alpha-Cardiac Actin antibody diluted at 1:1000.

Requested From: United States
Date Requested: 8/24/2016

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