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Anti-ACO1 / Aconitase Antibody (aa30-44) LS-C112852

Ordering

Wt. Vol. Conc. Price
100 µg - 0.5 mg/ml $395
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

Most Popular ACO1 / Aconitase Antibodies

Anti-ACO1 / Aconitase Antibody (aa106-155) LS-C117970
Rabbit Polyclonal (IgG) to Human ACO1 / Aconitase
Human, Mouse, Rat
IHC - Paraffin, Immunofluorescence, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ACO1 / Aconitase Antibody (clone 2C1) LS-C196600
Mouse Monoclonal [clone 2C1] (IgG2a,k) to Human ACO1 / Aconitase
Human
Western blot, Immunoprecipitation, ELISA
Unconjugated
Immunoprecipitation Image

100% Guaranteed 100% Guaranteed
Goat Polyclonal to Human ACO1 / Aconitase
Human, Monkey, Mouse, Rat, Rabbit
Western blot, ELISA
Unconjugated

Details

Human ACO1 / Aconitase
Goat
Human, Monkey, Mouse, Rat, Rabbit (tested or 100% immunogen sequence identity)
Bat, Bovine, Dog, Hamster, Horse, Chicken, Xenopus (at least 90% immunogen sequence identity)
Polyclonal
Unconjugated
Immunoaffinity purified
Unmodified

Applications

  • Western blot (0.1 - 0.3 µg/ml)
  • ELISA (1:8000)

Specificity and Use

ACO1 / Aconitase antibody was raised against synthetic peptide C-QVDFNRRADSLQKNQ from an internal region of human ACO1 (NP_002188.1). Percent identity by BLAST analysis: Human, Gorilla, Orangutan, Gibbon, Monkey, Mouse, Rat, Rabbit (100%); Hamster, Panda, Bovine, Bat, Dog, Horse, Opossum, Turkey, Chicken, Platypus, Xenopus (93%); Salmon, Zebrafish (87%); Stickleback (80%).
LS-E27112 - Lyophilized - 100 µg - $145.00
aa30-44
Human ACO1.
Peptide ELISA: antibody detection limit dilution 1:8000. Western blot: Approx 100kDa band observed in Human Liver lysates (calculated MW of 98.4kDa according to NP_002188.1). Recommended concentration: 0.1-0.3 ug/ml.

Packaging

Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Store at -20°C. Minimize freezing and thawing.
For research use only.

About ACO1 / Aconitase

P21399 NM_002197 NP_002188.1

ACO1 Antibody, ACONS Antibody, Aconitase Antibody, Aconitase 1, soluble Antibody, Aconitate hydratase Antibody, Citrate hydro-lyase Antibody, IREB1 Antibody, IRE-BP 1 Antibody, IREBP Antibody, IRP1 Antibody, Ferritin repressor protein Antibody, IREBP1 Antibody, Iron regulatory protein 1 Antibody

IRP1 / Aconitase is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate.

Western blot

Western blot
ACO1 antibody (0.1 ug/ml) staining of Human Liver lysate (35 ug protein/ml in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
 

Requested From: United States
Date Requested: 12/8/2016

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