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Anti-ACLY / ATP Citrate Lyase Antibody (clone 5F8D11) LS-C169149

Ordering

Wt. Vol. Conc. Price
100 µg - 1 mg/ml $365
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
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Most Popular ACLY / ATP Citrate Lyase Antibodies

Anti-ACLY / ATP Citrate Lyase Antibody (clone 5F8D11) LS-C169149
Mouse Monoclonal [clone 5F8D11] (IgG1) to Human ACLY / ATP Citrate Lyase
Human, Monkey, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated
Immunohistochemistry Image
Anti-ACLY / ATP Citrate Lyase Antibody (clone 3G8) IHC-plus™ LS-B9467
Mouse Monoclonal [clone 3G8] (IgG1) to Human ACLY / ATP Citrate Lyase
Human
IHC - Paraffin, Immunofluorescence, Western blot
Unconjugated
Immunohistochemistry Image

100% Guaranteed 100% Guaranteed
Mouse Monoclonal [clone 5F8D11] (IgG1) to Human ACLY / ATP Citrate Lyase
Human, Monkey, Mouse
IHC - Paraffin, ICC, Immunofluorescence, Western blot, Flow Cytometry, ELISA
Unconjugated

Details

Human ACLY / ATP Citrate Lyase
Mouse
Human, Monkey, Mouse (tested or 100% immunogen sequence identity)
IgG1 Monoclonal [5F8D11]
Unconjugated
Purified
Unmodified

Applications

  • IHC - Paraffin (1:200 - 1:1000)
  • ICC (1:50)
  • Immunofluorescence
  • Western blot (1:500 - 1:2000)
  • Flow Cytometry (1:200 - 1:400)
  • ELISA (1:10000)

Specificity and Use

ACLY / ATP Citrate Lyase antibody was raised against purified recombinant fragment of human ACLY (aa306-502 ) expressed in E. coli.
Human ACLY / Citrate Lyase

Packaging

PBS, 0.05% sodium azide, 0.05% protein stabilizer
Long term: -20°C; Short term: +4°C; Avoid freeze-thaw cycles.
For research use only.

About ACLY / ATP Citrate Lyase

P53396 NM_001096 NP_001087.2

ACLY Antibody, ACL Antibody, ATP citrate synthase Antibody, ATPCL Antibody, ATP citrate lyase Antibody, ATP-citrate (pro-S-)-lyase Antibody, CLATP Antibody, ATP-citrate synthase Antibody, Citrate cleavage enzyme Antibody, Citrate Lyase Antibody

ATP citrate lyase is the primary enzyme responsible for the synthesis of cytosolic acetyl-CoA in many tissues. The enzyme is a tetramer (relative molecular weight approximately 440,000) of apparently identical subunits. It catalyzes the formation of acetyl-CoA and oxaloacetate from citrate and CoA with a concomitant hydrolysis of ATP to ADP and phosphate. The product, acetyl-CoA, serves several important biosynthetic pathways, including lipogenesis and cholesterogenesis.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded esophageal cancer tissues using ACLY mouse monoclonal antibody with DAB staining.

Immunohistochemistry

Immunohistochemistry
IHC of paraffin-embedded endometrial cancer tissues using ACLY mouse monoclonal antibody with DAB staining.

Immunofluorescence

Immunofluorescence
Immunofluorescence of HeLa cells using ACLY mouse monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

Western blot

Western blot
Western blot using ACLY monoclonal antibody against human ACLY recombinant protein. (Expected MW is 46.7 kDa)

Western blot

Western blot
Western blot using ACLY mouse monoclonal antibody against HeLa (1), NIH3T3 (2), C6 (3), COS7 (4), and Raji (5) cell lysate.

Enzyme-Linked Immunosorbent Assay

Enzyme-Linked Immunosorbent Assay
Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Requested From: United States
Date Requested: 12/4/2016

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