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Anti-Acetyl-Lysine Antibody LS-C71875

Ordering

Wt. Vol. Conc. Price
- 100 µl - $650
Inquire for larger quantities

LSBio (Direct) LSBio (Direct)
206-374-1102
866-206-6909
Orders@LSBio.com
 

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Most Popular Acetyl-Lysine Antibodies

Anti-Acetyl-Lysine Antibody LS-C71873
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Anti-Acetyl-Lysine Antibody (clone 7F8, Atto 655) LS-C231003
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Immunofluorescence Image

100% Guaranteed 100% Guaranteed
Rabbit Polyclonal (IgG)
Western blot, Immunoprecipitation
Unconjugated

Details

Acetyl-Lysine
Rabbit
IgG Polyclonal
Unconjugated
Antiserum
Unmodified

Applications

  • Western blot
  • Immunoprecipitation (1:1000 - 1:2000)

Specificity and Use

Acetyl-Lysine antibody was raised against a mixture of chemically acetylated antigens.
Recognizes acetyl-lysine containing proteins including histones, from sodium butyrate or Trichostatin A treated cells, p53 acetylated in vitro by p300 or PCAF, and chemically acetylated BSA.
Suitable for use in Western Blot and Immunoprecipitation. Western Blot Analysis: 1:1000-1:2000 dilution of this lot detected: acetylated histones in acid-extracted proteins from sodium butyrate (5mM) treated HeLa cells. Sodium butyrate, an inhibitor of deacetylases, was used to increase the level of acetylated histones. (Panel A). Chemically acetylated BSA (10ng) in a dot blot assay. A 1:1000 dilution of the previous lot of the antibody detected: acetylated histones and to a lesser degree, other proteins in RIPA lysate from trichostatin A (5uM) treated Cos-1 cells. Trichostatin A, an inhibitor of deacetylases also, was used to increase the level of acetylated histones. (Panel B). GST-p53 (100ng) acetylated in vitro with either recombinant p300 or PCAF. (Panel C). Immunoprecipitation: 5 ul of a previous lot immunoprecipitated in vitro acetylated recombinant p300.

Packaging

0.05% sodium azide, 30% glycerol.
For research use only.

About Acetyl-Lysine


Requested From: United States
Date Requested: 12/7/2016

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