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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AANAT Antibody (DY550) LS‑C140755
AANAT antibody LS-C140755 is a DY550-conjugated rabbit polyclonal antibody to AANAT from human and primate. Validated for ICC, IHC and WB.
Catalog
Size
Price
LS-C140755-100
100 µl
Unavailable

Popular AANAT Products

AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Primate
Applications: IHC, Western blot
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Primate
Applications: IHC, ICC, Western blot
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
Species: Human, Primate
Applications: IHC, ICC, Western blot
Immunohistochemistry (IHC) analysis of AANAT antibody in paraffin-embedded human liver carcinoma tissue at 1:50, showing cytoplasmic strong positive staining. Negative control (the right) using PBS instead of primary antibody. Secondary antibody is Goat Anti-Rabbit IgG.
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot

Product Description

AANAT antibody LS-C140755 is a DY550-conjugated rabbit polyclonal antibody to AANAT from human and primate. Validated for ICC, IHC and WB.
About AANAT
AANAT belongs to the acetyltransferase superfamily. It is the penultimate enzyme in melatonin synthesis and controls the night/day rhythm in melatonin production in the vertebrate pineal gland. Melatonin is essential for the function of the circadian clock that influences activity and sleep. This enzyme is regulated by cAMP-dependent phosphorylation that promotes its interaction with 14-3-3 proteins and thus protects the enzyme against proteasomal degradation. Q16613 NM_001088 NP_001079.1

AANAT Antibody, AA-NAT Antibody, DSPS Antibody, Serotonin N-acetyltransferase Antibody, SNAT Antibody, Serotonin acetylase Antibody


Specifications

Target
Human AANAT
Host
Rabbit
Reactivity
Human, Primate (tested or 100% immunogen sequence identity)
Non-Reactivity
Rat, Bovine, Chicken, Zebrafish
Clonality
Polyclonal
Conjugations
DyLight 550. Also available Unconjugated or conjugated with DY650, DY488, Biotin.
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • ICC (1:200)
  • Western blot (1:500 - 1:1000)
  • (applications tested for the base form of this product only)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AANAT antibody was raised against human AANAT
Specificity
Primate AANAT (3,4).
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
50 mM sodium borate
Storage
Store at 4°C. Do not freeze.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Immunohistochemistry

AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
AANAT in COS7 and 1E7 cells detected by ICC.. Cells were grown on two-well chamber slides and fixed with 2.5% glutaraldehyde; 1E7 cells treated with FSK for 3 h (two magnifications). COS, COS7 cells treated with FSK for 3 h. This image was taken for the unconjugated form of this product. Other forms have not been tested.

Western blot

WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein.  This image was taken for the unconjugated form of this product. Other forms have not been tested.
WB: AANAT Antibody - Samples of 1E7 homogenates and bacterially expressed hAANAT were loaded, which contained similar levels of AANAT activity in 65 ug and 2.6 ng of protein. This image was taken for the unconjugated form of this product. Other forms have not been tested.


Requested From: 
Date Requested: 6/23/2018

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