Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to
worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting
immunostaining in relation to complex human pathologies.
TCR Screening Services
Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding.
Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".
Human, Mouse, Rat, Bovine, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Western blot (1:150 - 1:300)
Specificity and Use
Synthetic phospho peptide
14-3-3 phospho Serine185. By Western blot the antibody recognizes the ~30 kD protein. Specific for the phosphorylated form of beta and zeta 14-3-3 that are found endogenously phosphorylated only in brain tissue
Western blot: 1:150-1:300 using ECL. Optimal working dilutions must be determined by end user.
10 mM Tris-HCl buffer, pH 7.5, 150 mM sodium chloride, 100 ug/mL BSA, 50% glycerol, 0.05% sodium azide
Store at -20°C. Aliquot to avoid freeze/thaw cycles. Store undiluted.