14-3-3 antibody LS-B6644 is an unconjugated rabbit polyclonal antibody to 14-3-3 from human, mouse, rat and other species. Validated for IHC, IP and WB. Tested on 20 paraffin-embedded human tissues.
Sheep, Human, Mouse, Rat, Bovine, Chicken, Amphibian, Fungus, Plants, Reptile
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:200)
- Western blot (1:2000)
See our complete line of Immunohistochemistry Reagents
including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
14-3-3 antibody was raised against A mixture of synthetic peptides corresponding to all isoforms of sheep 14-3-3
Recognizes all isoforms of the 14-3-3 protein family. The 14-3-3 family consists of 30 kD proteins involved in multiple cellular processes including protein kinase signalling pathways, regulation of cell cycle progression, transcription and intracellular trafficking. Protein interactions with 14-3-3 show distinct preferences for its different isoforms and are regulated by phosphorylation of both 14-3-3 and the bound protein.
Western Blot: Detects a band of approximately 30kD in HEK293 cell lysates. Immunohistology: This product does not require antigen retrieval using heat treatment prior to staining of paraffin sections.
Serum, 0.09% Sodium Azide
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
For research use only.