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Catalog Number Size Price
LS-C18983-100 100 µg (1 mg/ml) $498 
ANAPC1 / APC1 Antibody - Anti-APC1 pS355 Antibody - Western Blot. Western blot of Affinity Purified anti-APC1 pS355 antibody shows detection of a band ~215 kD corresponding to phosphorylated human APC1 (arrowhead). Lane 1 shows lysate from asynchronous cells. Lane 2 shows lysate from cells treated with thymidine to synchronize cells at the G1/S boundary. Lane 3 shows lysate from cells treated with nocodazole to synchronize cells at the M phase. Phosphorylated APC1 is mostly present only in cell preparations arrested at cell division. Each lane contains approximately 30 ug of HeLa S3 whole cell lysates separated by 12.5% SDS-PAGE followed by transfer to nitrocellulose. After blocking with 5% non-fat dry milk in TTBS, the membrane was probed with the primary antibody diluted to 1:500 for 1 h at room temperature followed by washes and reaction with a 1:5000 dilution of HRP Gt-a-Rabbit IgG [H&L] MX (LS-C60865) for 45 min at room temperature. ECL reagent was used for detection. Other detection systems will yield similar results. Data contributed by Bing Li, UT Southwestern.

Polyclonal Rabbit anti‑Human ANAPC1 / APC1 Antibody (phospho‑Ser355, WB) LS‑C18983

Polyclonal Rabbit anti‑Human ANAPC1 / APC1 Antibody (phospho‑Ser355, WB) LS‑C18983

Antibody:
ANAPC1 / APC1 Rabbit anti-Human Polyclonal (pSer355) Antibody
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C18983-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ANAPC1 / APC1 Rabbit anti-Human Polyclonal (pSer355) Antibody
Application:
WB, IP, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Dog
Format:
Unconjugated, Unmodified

Specifications

Description
APC1 antibody LS-C18983 is an unconjugated rabbit polyclonal antibody to APC1 (ANAPC1) (pSer355) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IP and WB.
Target
Human ANAPC1 / APC1
Synonyms
ANAPC1 | APC1 | Cyclosome subunit 1 | MCPR | Mitotic checkpoint regulator | TSG24
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Dog (tested or 100% immunogen sequence identity)
Predicted
Chimpanzee, Mouse, Rat, Bovine, Dog, Chicken (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc1 protein.
Epitope
pSer355
Specificity
This antibody is specific for phosphorylated human APC1 protein at the pS355 residue. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from chimpanzee and chicken is expected as the sequence of the immunogen only varies by one amino acid in from these sources (89% homology). Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
Applications
  • Western blot (1:200 - 1:2000)
  • Immunoprecipitation (1:100)
  • ELISA (1:10000 - 1:35000)
Usage
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band - 215 kD in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ANAPC1 / APC1
Q9H1A4 NM_022662 NP_073153.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/11/2022