Human ANAPC1 / APC1
ANAPC1 | APC1 | Cyclosome subunit 1 | MCPR | Mitotic checkpoint regulator | TSG24
Human, Mouse, Rat, Bovine, Dog
(tested or 100% immunogen sequence identity)
Chimpanzee, Mouse, Rat, Bovine, Dog, Chicken (at least 90% immunogen sequence identity)
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc1 protein.
This antibody is specific for phosphorylated human APC1 protein at the pS355 residue. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, dog, rat, and bovine based on 100% homology for the immunogen sequence. Cross reactivity with APC1 protein from chimpanzee and chicken is expected as the sequence of the immunogen only varies by one amino acid in from these sources (89% homology). Cross reactivity with APC1 homologues from other sources has not been determined. Minimal reactivity is expected with the non-phosphorylated form of the protein.
- Western blot (1:200 - 1:2000)
- Immunoprecipitation (1:100)
- ELISA (1:10000 - 1:35000)
This affinity purified antibody has been tested for use in ELISA and western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band - 215 kD in size corresponding to APC1 by western blotting in the appropriate cell lysate or extract.
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Store vial at -20°C prior to opening. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
For research use only. Intended for use by laboratory professionals.
This antibody carries the LSBio 100% Guarantee.