Amphiphysin antibody LS-C111652 is an unconjugated rabbit polyclonal antibody to Amphiphysin (AMPH) from human, mouse, rat and other species. Validated for WB.
Human, Monkey, Mouse, Rat, Bovine, Horse, Rabbit, Chicken, Xenopus
(tested or 100% immunogen sequence identity)
Also available conjugated with Biotin
Synthetic peptide from N-Terminus of human AMPH (P49418, NP_001626). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Bovine, Rabbit, Horse, Zebra finch, Chicken, Lizard, Xenopus (100%); Galago, Dog, Pig, Opossum, Turkey, Stickleback, Zebrafish (92%); Salmon (90%).
Western Blot: Suggested dilution at 1 ug/ml in 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as secondary antibody.
PBS, 0.09% sodium azide, 2% sucrose
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.