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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AMPH / Amphiphysin Antibody (aa159‑170) IHC‑plus™ LS‑B2760
Note: This antibody replaces LS-C34668, LS-C68934
Amphiphysin antibody LS-B2760 is an unconjugated goat polyclonal antibody to Amphiphysin (AMPH) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
Catalog
Size
Price
LS-B2760-50
50 µg (0.5 mg/ml)
Unavailable

Popular AMPH / Amphiphysin Products

Antibody
Species: Human, Mouse, Rat
Applications: IHC, ICC, Western blot, Immunoprecipitation
Anti-AMPH / Amphiphysin antibody IHC of human small intestine, submucosal plexus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B3757 concentration 10 ug/ml.
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Hamster, Rabbit
Applications: IHC, IHC - Paraffin, Western blot, Immunoprecipitation
Anti-Amphiphysin antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B7822 dilution 1:200.
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, ICC, Western blot, Immunoprecipitation
Antibody
Species: Human
Applications: Western blot, ELISA

Product Description

Amphiphysin antibody LS-B2760 is an unconjugated goat polyclonal antibody to Amphiphysin (AMPH) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.
About AMPH / Amphiphysin
AMPH / Amphiphysin is a protein associated with the cytoplasmic surface of synaptic vesicles. A subset of patients with stiff-man syndrome who were also affected by breast cancer are positive for autoantibodies against this protein. Alternate splicing of this gene results in two transcript variants encoding different isoforms. Additional splice variants have been described, but their full length sequences have not been determined. A pseudogene of this gene is found on chromosome 11. P49418 NM_001635 NP_001626.1

AMPH Antibody, AMPH1 Antibody, Amphiphysin Antibody, Amphiphysin I Antibody


Specifications

Target
Human AMPH / Amphiphysin
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Predicted
Xenopus (at least 90% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Synthetic peptide C-KRKDESRISKAE from an internal region of human AMPH / Amphiphysin (NP_001626.1; NP_647477.1). Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Hamster, Elephant, Panda, Bovine, Dog, Bat, Horse, Rabbit (100%); Xenopus (92%); Opossum, Chicken, Lizard (83%).
Blocking Peptide
LS-E26093 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2760. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa159-170
Specificity
Human AMPH / Amphiphysin. This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
Usage
Immunohistochemistry: LS-B2760 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2760 was determined to be 2.5 ug/ml.
Presentation
Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.

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Images


Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.

Western blot

Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.

Western blot

Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.

Western blot

Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemistry

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody LS-B2760 concentration 5 ug/ml.

Western blot

Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.


Requested From: 
Date Requested: 6/24/2018

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