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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AMPH / Amphiphysin Antibody (aa159-170) IHC-plus™ LS-B2760

Note: This antibody replaces LS-C34668, LS-C68934
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Antibody (0.5 ug/ml) staining of mouse brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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AMPH / Amphiphysin Antibody (aa159-170) IHC-plus™ LS-B2760

Note: This antibody replaces LS-C34668, LS-C68934
Antibody:
Goat Polyclonal to Human AMPH / Amphiphysin
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-B2760-50
Toll Free North America
866-819-4732

Overview

Antibody:
Goat Polyclonal to Human AMPH / Amphiphysin
Application:
IHC, IHC-P, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit
Format:
Unconjugated, Unmodified

Specifications

Description
Amphiphysin antibody LS-B2760 is an unconjugated goat polyclonal antibody to Amphiphysin (AMPH) from human, mouse, rat and other species. Validated for IHC, Peptide-ELISA and WB. Tested on 20 paraffin-embedded human tissues.

Target
Human AMPH / Amphiphysin
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Hamster, Horse, Rabbit (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (2.5 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Peptide with sequence C-KRKDESRISKAE, from the internal region of the protein sequence according to NP_001626.1NP_647477.1.
Blocking Peptide
LS-E26093 - Lyophilized - 100 µg - $145.00
Immunizing peptide used to generate LS-B2760. Useful for pre-absorption and neutralization of the antibody's antigen binding site.
Epitope
aa159-170
Specificity
Human AMPH / Amphiphysin. This antibody is expected to recognise both reported isoforms (NP_001626.1 and NP_647477.1).
Usage
Immunohistochemistry: LS-B2760 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2760 was determined to be 2.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AMPH / Amphiphysin
P49418 NM_001635 NP_001626.1

Validation

Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
IHC Validated
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-AMPH / Amphiphysin antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.

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Reactivity: All species
Range: 0.78-50 ng/ml
Reactivity: Mouse
Range: 15.6-1000 pg/ml
Reactivity: Human
Range: 0.156-10 ng/ml
Reactivity: Human
Range: 12.35-1000 pg/ml

Requested From: United States
Date Requested: 7/18/2019
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