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| Catalog Number | Size | Price |
|---|---|---|
| LS-C209267-100 | 100 µg | $567 |
Polyclonal Sheep anti‑Bovine ALPI / Alkaline Phosphatase Antibody (Lyophilized, WB) LS‑C209267
Polyclonal Sheep anti‑Bovine ALPI / Alkaline Phosphatase Antibody (Lyophilized, WB) LS‑C209267
Antibody:
ALPI / Alkaline Phosphatase Sheep anti-Bovine Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Lyophilized
Toll Free North America
206-374-1102
206-374-1102For Research Use Only
Overview
Antibody:
ALPI / Alkaline Phosphatase Sheep anti-Bovine Polyclonal (Lyophilized) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Unconjugated, Lyophilized
Specifications
Description
Alkaline Phosphatase antibody LS-C209267 is an unconjugated sheep polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
Target
Bovine ALPI / Alkaline Phosphatase
Synonyms
ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
Host
Sheep
Reactivity
Bovine
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Alkaline Phosphatase [Calf intestine].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:4000 - 1:20000)
Usage
ALKALINE PHOSPHATASE antibody has been assayed against 1.0 ug of Alkaline Phosphatase [Calf Intestine] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:1000 to 1:5000 of the reconstitution concentration is suggested for this product.
Presentation
Lyophilized from 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
About ALPI / Alkaline Phosphatase
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Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
Date Requested: 4/28/2024
