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Catalog Number Size Price
LS-C745122-25 25 µl (1 mg/ml) $275 
ALPI / Alkaline Phosphatase Antibody - Western Blot of rabbit anti-Alkaline Phosphatase antibody Biotin Conjugated. Lane 1: Alkaline Phosphatase (calf intestine). Load: 50 ng per lane. Primary antibody: Alkaline Phosphatase antibody Biotin conjugated at 1:2,000 for 60 min at RT. Secondary antibody: Peroxidase Streptavidin secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting (MB-070) for 30 min at RT. Predicted/Observed size: 57 kDa, 57 kDa for Alkaline Phosphatase.

Polyclonal Rabbit anti‑Bovine ALPI / Alkaline Phosphatase Antibody (Liquid, Biotin, WB) LS‑C745122

Polyclonal Rabbit anti‑Bovine ALPI / Alkaline Phosphatase Antibody (Liquid, Biotin, WB) LS‑C745122

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Bovine Polyclonal (Liquid) (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Biotin, Liquid
Price
Catalog Number
$275
LS-C745122-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ALPI / Alkaline Phosphatase Rabbit anti-Bovine Polyclonal (Liquid) (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Bovine
Format:
Biotin, Liquid

Specifications

Description
Alkaline Phosphatase antibody LS-C745122 is a biotin-conjugated rabbit polyclonal antibody to bovine Alkaline Phosphatase (ALPI). Validated for ELISA and WB.
Target
Bovine ALPI / Alkaline Phosphatase
Synonyms
ALPI | Alkaline phosphomonoesterase | Kasahara isozyme | Glycerophosphatase | Alkaline Phosphatase | IAP
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Alkaline Phosphatase [Calf intestine].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine]. Cross reactivities against Alkaline Phosphatase from other sources may occur but have not been specifically determined.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:1000 - 1:10000)
Usage
Applications should be user optimized.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ALPI / Alkaline Phosphatase
P09923 NM_001631 NP_001622.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021