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Antibodies > Alpha Tubulin > LS-C355072
 
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Catalog Number Size Price
LS-C355072-50 50 µg $504 
Alpha Tubulin Antibody - Immunohistochemistry analysis of Alpha-Tubulin showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Alpha-Tubulin diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Alpha Tubulin Antibody - Immunohistochemistry analysis of Alpha-Tubulin showing staining in the cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Alpha-Tubulin diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Alpha Tubulin Antibody - IF using alpha-Tubulin Antibody, Biotin, Biotin conjugate (236-10501)
Alpha Tubulin Antibody - IF using alpha-Tubulin Antibody, Biotin, Biotin conjugate (236-10501)
Alpha Tubulin Antibody - IF using alpha-Tubulin Antibody, Biotin, Biotin conjugate (236-10501)
Alpha Tubulin Antibody - Western blot analysis of alpha-Tubulin was performed by loading 20 µg of HCT 116 (lane1), MDA-MB-231 (lane2), Raji (lane3) and A431 (lane4) cell lysate using 12 % Bis-Tris gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4°C overnight. alpha-Tubulin was detected at ~ 52 kDa using alpha-Tubulin Biotin Conjugated Mouse Monoclonal Antibody at 1:3000 dilution in 5% skim milk for 3 hour at room temperature on a rocking platform. Streptavidin - HRP at 1:2000 dilution was used .
Alpha Tubulin Antibody - Immunohistochemistry analysis of Alpha-Tubulin showing staining in the cytoplasm of paraffin-embedded mouse brain tissue (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Alpha-Tubulin diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Alpha Tubulin Antibody - Immunohistochemistry analysis of Alpha-Tubulin showing staining in the cytoplasm of paraffin-embedded human breast carcinoma (right) compared to a negative control without primary antibody (left). To expose target proteins, antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Following antigen retrieval, tissues were blocked in 3% H2O2-methanol for 15 min at room temperature, washed with ddH2O and PBS, and then probed with a Alpha-Tubulin diluted in 3% BSA-PBS at a dilution of 1:20 overnight at 4°C in a humidified chamber. Tissues were washed extensively in PBST and detection was performed using an HRP-conjugated secondary antibody followed by colorimetric detection using a DAB kit. Tissues were counterstained with hematoxylin and dehydrated with ethanol and xylene to prep for mounting.
Alpha Tubulin Antibody - IF using alpha-Tubulin Antibody, Biotin, Biotin conjugate (236-10501)
Alpha Tubulin Antibody - IF using alpha-Tubulin Antibody, Biotin, Biotin conjugate (236-10501)
Alpha Tubulin Antibody - IF using alpha-Tubulin Antibody, Biotin, Biotin conjugate (236-10501)
Alpha Tubulin Antibody - Western blot analysis of alpha-Tubulin was performed by loading 20 µg of HCT 116 (lane1), MDA-MB-231 (lane2), Raji (lane3) and A431 (lane4) cell lysate using 12 % Bis-Tris gel. Proteins were transferred to a nitrocellulose membrane and blocked with 5% skim milk at 4°C overnight. alpha-Tubulin was detected at ~ 52 kDa using alpha-Tubulin Biotin Conjugated Mouse Monoclonal Antibody at 1:3000 dilution in 5% skim milk for 3 hour at room temperature on a rocking platform. Streptavidin - HRP at 1:2000 dilution was used .
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Monoclonal Mouse anti‑Bovine Alpha Tubulin Antibody (clone 236‑10501, Biotin, IHC, IF, WB) LS‑C355072

Monoclonal Mouse anti‑Bovine Alpha Tubulin Antibody (clone 236‑10501, Biotin, IHC, IF, WB) LS‑C355072

Submit Review LSBio Guarantee
Antibody:
Alpha Tubulin Mouse anti-Bovine Monoclonal (Biotin) (236-10501) Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Bovine, Human, Mouse
Format:
Biotin, Unmodified
Price
Catalog Number
$504
LS-C355072-50
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Alpha Tubulin Mouse anti-Bovine Monoclonal (Biotin) (236-10501) Antibody
Application:
IHC, ICC, IF, WB
Reactivity:
Bovine, Human, Mouse
Format:
Biotin, Unmodified

Specifications

Description
Alpha Tubulin antibody LS-C355072 is a biotin-conjugated mouse monoclonal antibody to Alpha Tubulin from bovine. It is reactive with human, mouse and bovine. Validated for ICC, IF, IHC and WB.
Target
Bovine Alpha Tubulin
Host
Mouse
Reactivity
Bovine, Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
236-10501
Conjugations
Biotin
Purification
Purified
Modifications
Unmodified
Immunogen
Bovine Alpha-Tubulin.
Applications
  • IHC
  • ICC (1 µg/ml)
  • Immunofluorescence
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized No preservatives added
Reconstitution
Phosphate Buffered Saline (PBS)
Storage
Store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

Publications (0)

Submit a Publication for LS-C355072

Customer Reviews (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/27/2024
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