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Catalog Number Size Price
LS-C745296-25 25 µl (1.1 mg/ml) $275 
Alpha Tubulin Antibody - Immunofluorescence microscopy of rabbit Anti-alpha-Tubulin antibody using HeLa cells fixed with PFA. Anti-alpha-Tubulin Antibody was used at 1 µg/mL, O/N at 4°C. Secondary antibody: Anti-RABBIT IgG DyLight 488 Conjugated Preadsorbed at 2 ug/ml for 1 h at RT. Localization: TUBA1B is the major constituent of microtubules in the cytoplasm. Staining: Tubulin as green fluorescent signal with DAPI (blue) nuclear counterstain.
Alpha Tubulin Antibody - Western Blot of rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain. Lane 2: rat brain. Lane 3: A431 cells. Lane 4: Jurkat cells. Lane 5: HeLa cells. Load: 35 µg per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.
Alpha Tubulin Antibody - Western Blot of rabbit anti-alpha-Tubulin antibody. Lane 1: HeLa WCL. Lane 2: NIH/3T3 WCL. Load: 10 µg per lane. Primary antibody: alpha-Tubulin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for alpha-Tubulin. Other band(s): N/A.
Alpha Tubulin Antibody - ELISA results of purified Rabbit anti-alpha-Tubulin Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
Alpha Tubulin Antibody - Immunofluorescence microscopy of rabbit Anti-alpha-Tubulin antibody using HeLa cells fixed with PFA. Anti-alpha-Tubulin Antibody was used at 1 µg/mL, O/N at 4°C. Secondary antibody: Anti-RABBIT IgG DyLight 488 Conjugated Preadsorbed at 2 ug/ml for 1 h at RT. Localization: TUBA1B is the major constituent of microtubules in the cytoplasm. Staining: Tubulin as green fluorescent signal with DAPI (blue) nuclear counterstain.
Alpha Tubulin Antibody - Western Blot of rabbit Anti-Alpha Tubulin Antibody. Lane 1: whole cell lysates from mouse brain. Lane 2: rat brain. Lane 3: A431 cells. Lane 4: Jurkat cells. Lane 5: HeLa cells. Load: 35 µg per lane. Primary antibody: Alpha Tubulin antibody at 1:1,200 for overnight at 4°C. Secondary antibody: rabbit secondary antibody at 1:10,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: ~50 kDa corresponding to alpha tubulin (arrowhead). Other band(s): none.
Alpha Tubulin Antibody - Western Blot of rabbit anti-alpha-Tubulin antibody. Lane 1: HeLa WCL. Lane 2: NIH/3T3 WCL. Load: 10 µg per lane. Primary antibody: alpha-Tubulin antibody at 1:1,000 for overnight at 4°C. Secondary antibody: Peroxidase rabbit secondary antibody at 1:40,000 for 30 min at RT. Block: Blocking Buffer for Fluorescent Western Blotting for 30 min at RT. Predicted/Observed size: 50 kDa, 50 kDa for alpha-Tubulin. Other band(s): N/A.
Alpha Tubulin Antibody - ELISA results of purified Rabbit anti-alpha-Tubulin Antibody tested against BSA-conjugated peptide of immunizing peptide. Each well was coated in duplicate with 0.1µg of conjugate. The starting dilution of antibody was 5µg/ml and the X-axis represents the Log10 of a 3-fold dilution. This titration is a 4-parameter curve fit where the IC50 is defined as the titer of the antibody. Assay performed using 3% fish gel, Goat anti-Rabbit IgG Antibody Peroxidase Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins)
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Polyclonal Rabbit anti‑Human Alpha Tubulin Antibody (aa425‑451, IHC, IF, WB) LS‑C745296

Polyclonal Rabbit anti‑Human Alpha Tubulin Antibody (aa425‑451, IHC, IF, WB) LS‑C745296

Antibody:
Alpha Tubulin Rabbit anti-Human Polyclonal (aa425-451) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Chicken
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745296-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
Alpha Tubulin Rabbit anti-Human Polyclonal (aa425-451) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
Human, Mouse, Rat, Bovine, Chicken
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha Tubulin antibody LS-C745296 is an unconjugated rabbit polyclonal antibody to Alpha Tubulin (aa425-451) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA, IF, IHC and WB.
Target
Human Alpha Tubulin
Host
Rabbit
Reactivity
Human, Mouse, Rat, Bovine, Chicken (tested or 100% immunogen sequence identity)
Predicted
Fish, Algae, Avian, Bacteria, Mammal, Protozoa (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.
Epitope
aa425-451
Specificity
A BLAST analysis was used to suggest that this antibody would react with alpha Tubulin from a wide range of organisms, including avian, mammalian aquatic, parasitic and alga sources based on 100% homology for the immunogen sequence. Cross reactivity will occur with all isoforms of alpha tubulin. Such broad reactivity makes this antibody useful as an excellent loading control.
Applications
  • IHC (1:500 - 1:2000)
  • Immunofluorescence (1:500 - 1:2000)
  • Western blot (1:500 - 1:3000)
  • ELISA (1:5000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/14/2021