Rat Alpha-Fetoprotein

Sheep

Rat (tested or 100% immunogen sequence identity)

Polyclonal

Unconjugated. Also available conjugated with FITC.

Ion exchange chromatography

Unmodified

Rat alpha fetoprotein

Rat AFP / Alpha-Fetoprotein. The reactivity of the antiserum is restricted to alpha-fetoprotein. In immunoelectrophoresis and double radial immunodiffusion, using various antiserum concentrations against amniotic fluid, rat hepatoma sera and purified alpha-fetoprotein an single characteristic precipitin line is obtained. No reaction is obtained with rat sera of different strains. Cross-reactivity Inter-species cross-reactivity is a normal feature of antibodies to mammalian proteins, since homologous proteins of different species frequently share antigenic determinants. Cross-reactivity of this antiserum has not been tested in detail, however in double radial immunodiffusion (Ouchterlony) a precipitation reaction has been observed with mouse amniotic fluid.

• IHC - Paraffin (1:1000)
• Immunofluorescence
• Western blot
• ELISA

This product is intended for use as unlabelled primary or secondary antibody reagent for the indirect detection of alpha fetoprotein in tissues and body fluids in immunofluorescence and immunoenzyme methods; for the production of immunoconjugates with a selected marker; to prepare insoluble immunoaffinity adsorbents by coupling to an artificial carrier; as catching or detection reagent in non-isotopic methodology and solid phase immunochemistry (e. g. ELISA, Western blotting). When applied in any cytochemical or histochemical procedure or solids phase coupling technique, the optimum concentration of the IgG preparation should always be established by titration. Typical working dilutions in histochemistry are usually between 1:100 and 1:500; in ELISA and comparable non-precipitating antibody-binding assays between 1:1000 and 1:5000.

Lyophilized from PBS, pH 7.2

Use 1 ml of sterile Distilled water.

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P02771 NCBI: NM_001134 NP_001125.1