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Antibodies > Alpha-Fetoprotein > LS-C355401
 
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Catalog Number Size Price
LS-C355401-1 1 mg $857 
Alpha-Fetoprotein Antibody - Immunofluorescent analysis of alpha-fetoprotein (green) in the endoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute, WA09). After four days in suspension culture, EBs were plated on Geltrex-coated tissue culture-treated polystyrene plates and continuously cultured for 21 days. EB cultures were then fixed and permeabilized according to the 3-Germ Layer Immunocytochemistry Kit and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with anti-alpha-fetoprotein at 4°C overnight, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:1000 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342. Stained cells were imaged at 40X.
Alpha-Fetoprotein Antibody - Immunofluorescence analysis of alpha-fetoproprotein (AFP) (green) in the endoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute.) After four days in suspension culture, EBs were plated on Geltrex-coated tissue culture-treated polystyrene plates and continuously cultured for 21 days. EB cultures were then fixed and permeabilized according to the 3-Germ Layer Immunocytochemistry Kit and stained with anti-alpha-fetoprotein at 4°C overnight. Secondary staining was completed using Alexa Fluor 488-conjugated anti-mouse IgG and DAPI for nuclear DNA (blue) for 1 h at room temperature. Stained wells were imaged at 40X.
Alpha-Fetoprotein Antibody - Western blot analysis of alpha-fetoprotein was performed by loading 10 µg of whole cell extract from H9 ESCs, H9 ESC-derived hepatoblasts, ESC-derived hepatocyte-like cells, HepG2 cells, HepaRG cells, and primary human hepatocytes in reducing conditions and 10 ul Spectra Multicolor Broad Range Protein Ladder per well onto a 4-12% Bis-Tris gel. Proteins were transferred to a PVDF membrane, and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. alpha-Fetoprotein was detected at 67 kDa using an alpha-fetoprotein monoclonal antibody at a concentration of 1 µg/mL in 5% non-fat milk in TBST overnight at 4°C on a rocking platform, followed by a goat anti-mouse secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature.
Alpha-Fetoprotein Antibody - Western blot analysis of alpha-fetoprotein was performed by loading 20ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% BSA in TBST for 1 hour at room temperature. Alpha-fetoprotein was detected at ~67kDa using a alpha-fetoprotein mouse monoclonal antibody at a concentration of 2 µg/mL in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico.
Alpha-Fetoprotein Antibody - Western blot analysis of alpha-fetoprotein was performed by loading 40ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% BSA in TBST for 1 hour at room temperature. Alpha-fetoprotein was detected at ~67kDa using a alpha-fetoprotein mouse monoclonal antibody at a concentration of 2 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico.
Alpha-Fetoprotein Antibody - Immunofluorescent analysis of alpha-fetoprotein (green) in the endoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute, WA09). After four days in suspension culture, EBs were plated on Geltrex-coated tissue culture-treated polystyrene plates and continuously cultured for 21 days. EB cultures were then fixed and permeabilized according to the 3-Germ Layer Immunocytochemistry Kit and blocked with 3% BSA for 30 minutes at room temperature. Cells were stained with anti-alpha-fetoprotein at 4°C overnight, and then incubated with a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate at a dilution of 1:1000 for at least 30 minutes at a room temperature in the dark (green). Nuclei (blue) were stained with Hoechst 33342. Stained cells were imaged at 40X.
Alpha-Fetoprotein Antibody - Immunofluorescence analysis of alpha-fetoproprotein (AFP) (green) in the endoderm derived from human ES cells. Embryoid bodies (EBs) were generated from the H9 embryonic stem cell line (WiCell Research Institute.) After four days in suspension culture, EBs were plated on Geltrex-coated tissue culture-treated polystyrene plates and continuously cultured for 21 days. EB cultures were then fixed and permeabilized according to the 3-Germ Layer Immunocytochemistry Kit and stained with anti-alpha-fetoprotein at 4°C overnight. Secondary staining was completed using Alexa Fluor 488-conjugated anti-mouse IgG and DAPI for nuclear DNA (blue) for 1 h at room temperature. Stained wells were imaged at 40X.
Alpha-Fetoprotein Antibody - Western blot analysis of alpha-fetoprotein was performed by loading 10 µg of whole cell extract from H9 ESCs, H9 ESC-derived hepatoblasts, ESC-derived hepatocyte-like cells, HepG2 cells, HepaRG cells, and primary human hepatocytes in reducing conditions and 10 ul Spectra Multicolor Broad Range Protein Ladder per well onto a 4-12% Bis-Tris gel. Proteins were transferred to a PVDF membrane, and blocked with 5% non-fat milk in TBST for 1 hour at room temperature. alpha-Fetoprotein was detected at 67 kDa using an alpha-fetoprotein monoclonal antibody at a concentration of 1 µg/mL in 5% non-fat milk in TBST overnight at 4°C on a rocking platform, followed by a goat anti-mouse secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature.
Alpha-Fetoprotein Antibody - Western blot analysis of alpha-fetoprotein was performed by loading 20ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% BSA in TBST for 1 hour at room temperature. Alpha-fetoprotein was detected at ~67kDa using a alpha-fetoprotein mouse monoclonal antibody at a concentration of 2 µg/mL in blocking buffer for 1 hour at room temperature on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico.
Alpha-Fetoprotein Antibody - Western blot analysis of alpha-fetoprotein was performed by loading 40ug of the indicated whole cell lysates and 5ul of PageRuler Plus Prestained Protein Ladder per well onto a 4-20% Tris-Glycine polyacrylamide gel. Proteins were transferred to a nitrocellulose membrane using the G2 Blotter, and blocked with 5% BSA in TBST for 1 hour at room temperature. Alpha-fetoprotein was detected at ~67kDa using a alpha-fetoprotein mouse monoclonal antibody at a concentration of 2 µg/mL in blocking buffer overnight at 4C on a rocking platform, followed by a Goat anti-Mouse IgG (H+L) Superclonal Secondary Antibody, HRP conjugate at a dilution of 1:1000 for at least 30 minutes at room temperature. Chemiluminescent detection was performed using SuperSignal West Pico.
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Monoclonal Mouse anti‑Human Alpha‑Fetoprotein Antibody (clone P5B8, IF, WB) LS‑C355401

Monoclonal Mouse anti‑Human Alpha‑Fetoprotein Antibody (clone P5B8, IF, WB) LS‑C355401

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Antibody:
Alpha-Fetoprotein Mouse anti-Human Monoclonal (P5B8) Antibody
Application:
IF, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$857
LS-C355401-1
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
Alpha-Fetoprotein Mouse anti-Human Monoclonal (P5B8) Antibody
Application:
IF, WB, IP, ELISA, RIA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Alpha-Fetoprotein antibody LS-C355401 is an unconjugated mouse monoclonal antibody to human Alpha-Fetoprotein. Validated for ELISA, IF, IP, RIA and WB.
Target
Human Alpha-Fetoprotein
Synonyms
AFP | Alpha-1-fetoprotein | Alpha-fetoprotein | HPAFP | Alpha-fetoglobulin | FETA
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
P5B8
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
Purified AFP from cord blood.
Specificity
The antibody targets Alpha Fetoprotein in ELISA, IP, and RIA applications and shows reactivity with Human samples. It detects Alpha Fetoprotein which has a predicted molecular weight of approximately 67 kD.
Applications
  • Immunofluorescence
  • Western blot
  • Immunoprecipitation
  • ELISA
  • Radioimmunoassay
Presentation
PBS, pH 7.4, 0.09% Sodium Azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Alpha-Fetoprotein
P02771 NM_001134 NP_001125.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/30/2024
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