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Catalog Number Size Price
LS-C745157-50 50 µg (1 mg/ml) $492 
AKT2 Antibody - Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat secondary antibody anti rat at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.
AKT2 Antibody - Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
AKT2 Antibody - ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 unconjugated antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB
AKT2 Antibody - Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat secondary antibody anti rat at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.
AKT2 Antibody - Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 unconjugated antibody at 1:1000 for overnight at 4°C. Secondary antibody: Rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
AKT2 Antibody - ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 unconjugated antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB
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Monoclonal Rat anti‑Human AKT2 Antibody (clone 11F6.B2, FITC, Internal, IHC, IF, WB) LS‑C745157

Monoclonal Rat anti‑Human AKT2 Antibody (clone 11F6.B2, FITC, Internal, IHC, IF, WB) LS‑C745157

Antibody:
AKT2 Rat anti-Human Monoclonal (Internal) (FITC) (11F6.B2) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
FITC, Unmodified
Price
Catalog Number
$492
LS-C745157-50
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
AKT2 Rat anti-Human Monoclonal (Internal) (FITC) (11F6.B2) Antibody
Application:
IHC, IF, WB, Flo, ELISA
Reactivity:
Human
Format:
FITC, Unmodified

Specifications

Description
AKT2 antibody LS-C745157 is an FITC-conjugated rat monoclonal antibody to human AKT2 (Internal). Validated for ELISA, Flow, IF, IHC and WB.
Target
Human AKT2
Synonyms
AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a,k Monoclonal
Clone
11F6.B2
Conjugations
FITC
Purification
Protein G affinity chromatography
Modifications
Unmodified
Immunogen
Anti-AKT2 Antibody was produced in rat by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Epitope
Internal
Applications
  • IHC
  • Immunofluorescence
  • Western blot
  • Flow Cytometry
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.5 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 50µL of deionized water.
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT2
P31751 NM_001626 NP_001617.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021