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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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AKT2 Antibody LS‑C745153
AKT2 antibody LS-C745153 is an unconjugated rat monoclonal antibody to human AKT2. Validated for ELISA, IHC and WB.
Catalog
Size
Price
LS-C745153-25
25 µl
$235
Description
AKT2 antibody LS-C745153 is an unconjugated rat monoclonal antibody to human AKT2. Validated for ELISA, IHC and WB.
Target
Human AKT2
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Conjugations
Unconjugated
Modifications
Unmodified
Applications
  • IHC (20 µg/ml)
  • Western blot (1:500 - 1:2000)
  • ELISA (1:2000 - 1:10000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
AKT2 antibody was raised against anti-AKT2 Antibody was produced by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT2 protein.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.
Restrictions
For research use only.
About AKT2
P31751 NM_001626 NP_001617.1

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Images

Western blot

Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.
Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.

Western blot

Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.

Enzyme-Linked Immunosorbent Assay

ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB
ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB

Western blot

Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.
Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.

Western blot

Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.

Enzyme-Linked Immunosorbent Assay

ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB
ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB

Western blot

Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.
Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.

Western blot

Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.

Enzyme-Linked Immunosorbent Assay

ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB
ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB

Western blot

Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.
Western Blot of Rat monoclonal anti-AKT2 antibody. Lane 1: GST-AKT1. Lane 2: GST-AKT2. Lane 3: GST-AKT3. Load: 50 ng per lane. Primary antibody: anti-AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:40,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 85 kDa for GST-AKT2.

Western blot

Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.
Western Blot of Rat Anti-AKT2 antibody. Lane 1: LnCap lysate Lane 2: Jurkat lysate. Lane 3: MDA-MB 468 lysate. Load: 5 µg per lane. Primary antibody: AKT2 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rat secondary antibody at 1:20,000 for 45 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 56 kDa for AKT2.

Enzyme-Linked Immunosorbent Assay

ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB
ELISA of Rat Monoclonal anti-AKT2 antibody. Antigen: GST AKT1, GST AKT2, GST AKT3. Coating amount: starting from 50 ng/well. Primary antibody: Rat monoclonal anti-AKT2 antibody at 100 ng/well. Dilution series: 2-fold. Mid-point concentration: 3 ng/mL Rat monoclonal anti-AKT2 antibody. Secondary antibody: Peroxidase rat secondary antibody at 1:10,000. Substrate: TMB

Requested From: United States
Date Requested: 12/14/2018
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