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Catalog Number Size Price
LS-C19061-100 100 µg (1 mg/ml) $498 
AKT2 Antibody - Anti-AKT2 Antibody - Western Blot. Affinity Purified antibody to AKT2 was used at a 1:1000 dilution to detect AKT2 by Western blot of lysates from mouse tissues. The antibody is shown to react with mouse Akt2 in mouse liver, skeletal muscle and fat using standard western blotting methods.
AKT2 Antibody - Anti-AKT2 Antibody - Western Blot. Antibody to AKT2 detects both unphosphorylated and phosphorylated AKT2 in Western blot. As before approximately 20 mg/lane of crude HEK293 lysate was loaded for SDS-PAGE, separated and then transferred to nitrocellulose. The right lane contains lysate pretreated for 15min with 25 uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This antibody clearly detects both the phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2.
AKT2 Antibody - Anti-AKT2 Antibody - Western Blot. Anti-AKT2 Antibody in Western blot shows detection of AKT2. A lysate from a stable HEK293 cell line expressing an inducible, myristoylated form of Akt2 (MyrAkt2-ER) was loaded for SDS- PAGE, separated and then transferred to nitrocellulose. The blot was used at 1:1000 and reacted with Anti-AKT2 Antibody for 1h at room temperature. In response to 4OHT (tamoxifen), the Akt2 is recruited to the plasma membrane via its myristoylation sequence, and becomes phosphorylated and activated. The blot (right panel) shows 0 m, 15 m, and 30 m of 4OHT treatment. This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2. Endogenous Akt2 runs at about 60kD whereas the myristoylated construct runs at around 110kD. Antibody to AKT2 detects both unphosphorylated and phosphorylated AKT2. As before approximately 20 mg/lane of crude HEK293 lysate was loaded for SDS-PAGE, separated and then transferred to nitrocellulose. The right lane contains lysate pretreated-for-15min-with 25 uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This antibody clearly detects both the phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2.
AKT2 Antibody - Anti-AKT2 Antibody - Western Blot. Affinity Purified antibody to AKT2 was used at a 1:1000 dilution to detect AKT2 by Western blot of lysates from mouse tissues. The antibody is shown to react with mouse Akt2 in mouse liver, skeletal muscle and fat using standard western blotting methods.
AKT2 Antibody - Anti-AKT2 Antibody - Western Blot. Antibody to AKT2 detects both unphosphorylated and phosphorylated AKT2 in Western blot. As before approximately 20 mg/lane of crude HEK293 lysate was loaded for SDS-PAGE, separated and then transferred to nitrocellulose. The right lane contains lysate pretreated for 15min with 25 uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This antibody clearly detects both the phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2.
AKT2 Antibody - Anti-AKT2 Antibody - Western Blot. Anti-AKT2 Antibody in Western blot shows detection of AKT2. A lysate from a stable HEK293 cell line expressing an inducible, myristoylated form of Akt2 (MyrAkt2-ER) was loaded for SDS- PAGE, separated and then transferred to nitrocellulose. The blot was used at 1:1000 and reacted with Anti-AKT2 Antibody for 1h at room temperature. In response to 4OHT (tamoxifen), the Akt2 is recruited to the plasma membrane via its myristoylation sequence, and becomes phosphorylated and activated. The blot (right panel) shows 0 m, 15 m, and 30 m of 4OHT treatment. This treatment has no effect on endogenous Akt2, but causes a band shift upwards in the MyrAkt2. Endogenous Akt2 runs at about 60kD whereas the myristoylated construct runs at around 110kD. Antibody to AKT2 detects both unphosphorylated and phosphorylated AKT2. As before approximately 20 mg/lane of crude HEK293 lysate was loaded for SDS-PAGE, separated and then transferred to nitrocellulose. The right lane contains lysate pretreated-for-15min-with 25 uM LY294002 which affects the phosphorylation of endogenous Akt2 but has no effect on phosphorylation of the myristoylated construct. This antibody clearly detects both the phosphorylated (top arrow) and the non-phosphorylated (bottom arrow) forms of endogenous Akt2.
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Polyclonal Rabbit anti‑Human AKT2 Antibody (aa450‑475, WB) LS‑C19061

Polyclonal Rabbit anti‑Human AKT2 Antibody (aa450‑475, WB) LS‑C19061

Note: This antibody replaces LS-C3609
Antibody:
AKT2 Rabbit anti-Human Polyclonal (aa450-475) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$498
LS-C19061-100
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
AKT2 Rabbit anti-Human Polyclonal (aa450-475) Antibody
Application:
WB, Flo, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
AKT2 antibody LS-C19061 is an unconjugated rabbit polyclonal antibody to human AKT2 (aa450-475). Validated for ELISA, Flow and WB. Cited in 1 publication.
Target
Human AKT2
Synonyms
AKT2 | PKB beta | PKBBETA | PRKBB | Protein kinase Akt-2 | Protein kinase B beta | RAC-PK-beta | Rac protein kinase beta | RAC-BETA | HIHGHH | PKBB
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Predicted
Rat (at least 90% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-Terminal region near aa 450-475 (of 481) of human AKT2 conjugated to Keyhole Limpet Hemocyanin (KLH).
Epitope
aa450-475
Specificity
The antibody detects both unphosphorylated and phosphorylated forms of the protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. Cross reactivity with AKT2 from other species has not been determined, however, the sequence of the immunogen shows 85% identity to mouse and 92% identity with rat, therefore, cross reactivity is expected.
Applications
  • Western blot (1:1000)
  • Flow Cytometry
  • ELISA (1:4000 - 1:16000)
Usage
This antibody was tested by ELISA and immunoblotting and was found to be reactive with both unphosphorylated and phosphorylated AKT2 in a lysate of HEK293 cells. Although not tested, this antibody is likely functional in immunohistochemistry and immunoprecipitation. This product has been assayed by immunoblot against a HEK293 cell lysate and is reactive at a 1:1000 dilution showing a band at approximately 60 kD. A working dilution of 1:4000 to 1:16000 is suggested for this product in a standard capture ELISA using TMB as a substrate for 30 minutes at room temperature against 0.1 ug of the immunizing peptide. Researchers should determine optimal titers for other applications.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AKT2
P31751 NM_001626 NP_001617.1

LSBio Ratings

AKT2 Antibody (aa450-475) for WB/Western, Flow, ELISA LS-C19061 has an LSBio Rating of
Publications (4)

Publications (1)

Inhibition of protein kinase Akt1 by apoptosis signal-regulating kinase-1 (ASK1) is involved in apoptotic inhibition of regulatory volume increase. Subramanyam M, Takahashi N, Hasegawa Y, Mohri T, Okada Y. The Journal of biological chemistry. 2010 285:6109-17.

Customer Reviews (0)


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Requested From: United States
Date Requested: 8/15/2022