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Immunohistochemistry Services

Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

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Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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Cell Cycle Pathways
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Catalog Number Size Price
LS-C153762-100 100 µl (115 mg/ml) $425 
AHSP / EDRF Antibody - Immunohistochemistry of Rabbit anti-AHSP antibody. Tissue: A. Normal bone marrow, H&E. B. CD235a stains both nucleated EPs and mature, anucleate RBCs. C. AHSP stains nucleated EPs, but not mature, anucleate RBCs. D. CD71 stains nucleated EPs, but not mature, anucleate RBCs. Fixation: acetic acid-zinc-formalin and formalin fixation, embedded in paraffin Antigen retrieval: TRIS-EDTA pH9.0 Primary antibody: AHSP antibody at 1:8000 for overnight at 4°C Secondary antibody: anti-rabbit secondary at (1:10000 for 45 min at RT) Localization: Anti-AHSP is cytoplasmic Staining: AHSP antibody as precipitated brown signal with a purple nuclear counterstain using Bond-max fully automated for IHC.

AHSP / EDRF Antibody LS‑C153762

AHSP / EDRF Antibody LS‑C153762

Antibody:
Rabbit Polyclonal to Human AHSP / EDRF
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$425 
LS-C153762-100
100 µl
115 mg/ml
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to Human AHSP / EDRF
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
EDRF antibody LS-C153762 is an unconjugated rabbit polyclonal antibody to EDRF (AHSP ) from human and mouse. Validated for ELISA, IHC and WB. Cited in 1 publication.
Target
Human AHSP / EDRF
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Delipidated and defibrinated
Modifications
Unmodified
Applications
  • IHC
  • IHC - Paraffin (1:8000)
  • Western blot (1:500 - 1:1000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
Anti-AHSP Antibody was produced from whole rabbit serum prepared by repeated immunizations with the full length human AHSP protein.
Specificity
A BLAST analysis was used to suggest cross reactivity with human and mouse. Cross-reactivity with AHSP from other sources have not been determined.
Usage
Anti-AHSP Antibody is suitable for western blotting, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures and ELISA. Specific conditions for reactivity should be optimized by the end user.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
100 µl deionized water
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About AHSP / EDRF
Q9NZD4 NM_016633 NP_057717.1

Publications (1)

a-Hemoglobin-stabilizing protein is a sensitive and specific marker of erythroid precursors. Raess PW, Paessler ME, Bagg A, Weiss MJ, Choi JK. The American journal of surgical pathology. 2012 36:1538-47.

Customer Reviews (0)


Requested From: United States
Date Requested: 11/17/2019