RAGE antibody LS-C355523 is an unconjugated rabbit polyclonal antibody to RAGE (AGER) from human, mouse and rat. Validated for IF, IHC and WB.
Rat, Human, Mouse
(tested or 100% immunogen sequence identity)
- IHC - Paraffin (1:10 - 1:100)
- IHC - Frozen (1 - 2 µg/ml)
- Western blot (1 µg/ml)
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AGER / RAGE antibody was raised against synthetic peptides corresponding to residues C(362) W R K R Q P R (R/L) E E R K A P E S Q E D(380) of rat RAGE.
The antibody detects receptor for advanced glycation endproducts (RAGE) from mouse and rat samples. It has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects two bands in the 45 kD range representing the RAGE protein pre and post-glycosylation in mouse lung extract. This antibody also detects an ~25 kD protein that is believed to be proteolytic degradation product. Immunohistochemical staining of RAGE in transgenic mouse retina results in staining of the retinal pigmented epithelium and photo receptor cell layers. This sequence is 100% conserved in mouse and 70% conserved in humans.
PBS, 1 mg/ml BSA, 0.05% Sodium Azide
Store at -20°C. Avoid freeze-thaw cycles.
For research use only.