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Antibodies > ADAMTS13 > LS-C662315
 
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Catalog Number Size Price
LS-C662315-10 10 µg $318 
LS-C662315-100 100 µg $470 
ADAMTS13 Antibody - Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: rat brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS13 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAMTS13 at approximately 154KD. The expected band size for ADAMTS13 is at 154KD.
ADAMTS13 Antibody - Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody. Western blot analysis of ADAMTS13 expression in human HepG2 whole cell lysates 50ug, ADAMTS13 at 154KD under non-reducing conditions and 200 kD under reducing conditions were detected using rabbit anti-ADAMTS13 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
ADAMTS13 Antibody - Western blot - Anti-ADAMTS13 Picoband Antibody
ADAMTS13 Antibody - Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse liver tissue lysates, Lane 2: rat brain tissue lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADAMTS13 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADAMTS13 at approximately 154KD. The expected band size for ADAMTS13 is at 154KD.
ADAMTS13 Antibody - Western blot analysis of ADAMTS13 using anti-ADAMTS13 antibody. Western blot analysis of ADAMTS13 expression in human HepG2 whole cell lysates 50ug, ADAMTS13 at 154KD under non-reducing conditions and 200 kD under reducing conditions were detected using rabbit anti-ADAMTS13 Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.
ADAMTS13 Antibody - Western blot - Anti-ADAMTS13 Picoband Antibody
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Polyclonal Rabbit anti‑Human ADAMTS13 Antibody (WB) LS‑C662315

Polyclonal Rabbit anti‑Human ADAMTS13 Antibody (WB) LS‑C662315

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Antibody:
ADAMTS13 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662315-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ADAMTS13 Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ADAMTS13 antibody LS-C662315 is an unconjugated rabbit polyclonal antibody to human ADAMTS13. Validated for ELISA and WB.
Target
Human ADAMTS13
Synonyms
ADAMTS13 | ADAM-TS | ADAM-TS13 | ADAMTS-13 | ADAM-TS 13 | C9orf8 | TTP | VWF-CP | VWF-cleaving protease | VWFCP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human ADAMTS13 recombinant protein (Position: A299-R488). Human ADAMTS13 shares 85.2% amino acid (aa) sequence identity with mouse ADAMTS13.
Specificity
Plasma. Expressed primarily in liver. .
Applications
  • Western blot
  • ELISA
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADAMTS13
Q76LX8 NM_139025 NP_620594.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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