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Catalog Number Size Price
LS-C745050-100 100 µg (1 mg/ml) $492 
ADA / Adenosine Deaminase Antibody - Biotin conjugated anti adenosine deaminase was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.

Polyclonal Rabbit anti‑Bovine ADA / Adenosine Deaminase Antibody (Lyophilized, Biotin, WB) LS‑C745050

Polyclonal Rabbit anti‑Bovine ADA / Adenosine Deaminase Antibody (Lyophilized, Biotin, WB) LS‑C745050

Antibody:
ADA / Adenosine Deaminase Rabbit anti-Bovine Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
Bovine
Format:
Biotin, Lyophilized
Price
Catalog Number
$492
LS-C745050-100
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ADA / Adenosine Deaminase Rabbit anti-Bovine Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, IP, ELISA
Reactivity:
Bovine
Format:
Biotin, Lyophilized

Specifications

Description
Adenosine Deaminase antibody LS-C745050 is a biotin-conjugated rabbit polyclonal antibody to bovine Adenosine Deaminase (ADA). Validated for ELISA, IP and WB.
Target
Bovine ADA / Adenosine Deaminase
Synonyms
ADA | Adenosine deaminase | ADA1 | Adenosine aminohydrolase
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Adenosine Deaminase [Calf Spleen].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other sources may occur but has not been specifically determined.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:5000 - 1:20000)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADA / Adenosine Deaminase
P00813 NM_000022 NP_000013.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/11/2021