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Antibodies > ADA / Adenosine Deaminase > LS-C745034
 
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Catalog Number Size Price
LS-C745034-25 25 µl (1 mg/ml) $304 
ADA / Adenosine Deaminase Antibody - Western Blot of rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated. Lane 1: Adenosine Deaminase (Calf Spleen). Load: 50 ng per lane. Primary antibody: Rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 41 kDa, 45 kDa for Adenosine Deaminase (Calf Spleen).
ADA / Adenosine Deaminase Antibody - Biotin conjugated anti adenosine deaminase was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.
ADA / Adenosine Deaminase Antibody - Western Blot of rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated. Lane 1: Adenosine Deaminase (Calf Spleen). Load: 50 ng per lane. Primary antibody: Rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugated at 1:1,000 overnight at 4°C. Secondary antibody: n/a. Block: MB-070 for 30 min at RT. Predicted/Observed size: 41 kDa, 45 kDa for Adenosine Deaminase (Calf Spleen).
ADA / Adenosine Deaminase Antibody - Biotin conjugated anti adenosine deaminase was used to detect adenosine deaminase in mouse pancreas lysate (Left, Lane 1, 30 ul) under reducing conditions. The antibody was also used to detect purified Adenosine Deaminase (right, Lane 2), and endogenous Adenosine Deaminase in whole cell lysate from Jurkat and Raji cells (1:1 mixture, lane 3,) as well as Mouse Pancreas and Liver (1:1 mixture, lane 4). Lysates were run on 4-20% gel 140V under reducing conditions, transferred for 30 minutes at 100 V and blocked with 3% Fish Gel (left) or 3% BSA. Blot was incubated with 200-406-140 lot 5628 (1:5K in TBS, ON 4°C), washed 3X in TBS and incubated for 30 minutes with Dylight 488 conjugated Streptavidin (S000-41 lot 20833 1:5K in MB-070). Blot was imaged with the Biorad VersaDoc imaging system.
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Polyclonal Rabbit anti‑Bovine ADA / Adenosine Deaminase Antibody (Liquid, HRP, WB) LS‑C745034

Polyclonal Rabbit anti‑Bovine ADA / Adenosine Deaminase Antibody (Liquid, HRP, WB) LS‑C745034

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Antibody:
ADA / Adenosine Deaminase Rabbit anti-Bovine Polyclonal (Liquid) (HRP) Antibody
Application:
WB, IP, ELISA
Reactivity:
Bovine
Format:
Horseradish Peroxidase, Liquid
Price
Catalog Number
$304
LS-C745034-25
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ADA / Adenosine Deaminase Rabbit anti-Bovine Polyclonal (Liquid) (HRP) Antibody
Application:
WB, IP, ELISA
Reactivity:
Bovine
Format:
Horseradish Peroxidase, Liquid

Specifications

Description
Adenosine Deaminase antibody LS-C745034 is an HRP-conjugated rabbit polyclonal antibody to bovine Adenosine Deaminase (ADA). Validated for ELISA, IP and WB.
Target
Bovine ADA / Adenosine Deaminase
Synonyms
ADA | Adenosine deaminase | ADA1 | Adenosine aminohydrolase
Host
Rabbit
Reactivity
Bovine (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Liquid
Immunogen
Adenosine Deaminase [Calf Spleen] ADA
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen]. Cross reactivity against Adenosine Deaminase from other sources is unknown.
Applications
  • Western blot (1:500 - 1:5000)
  • Immunoprecipitation (1:100)
  • ELISA (1:1000 - 1:5000)
Usage
Applications should be user optimized.
Presentation
10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Storage
Store vial at 4°C prior to restoration. For extended storage aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles. Stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADA / Adenosine Deaminase
P00813 NM_000022 NP_000013.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/26/2024
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