Products
Research Areas
COVID-19
Resources
Contact Us
Login
Quick Order
Cart
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Cart
How To Buy
Contact Us

Locations

Orders Processing,
Shipping & Receiving,
Warehouse

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Production Lab

Floor 6, Suite 620
20700 44th Avenue W
Lynnwood, WA 98036

Telephone Numbers


Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

Contact Us


Additional Contact Details

Distributors
Login
Registration enables users to use special features of this website, such as past
order histories, retained contact details for faster checkout, review submissions, and special promotions.


Fields marked with a * are required.

Login
Quick Order
Antibodies > ADA / Adenosine Deaminase > LS-C756634
 
Print Page  
Catalog Number Size Price
LS-C756634-10 10 µg $318 
LS-C756634-100 100 µg $470 
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - Western blot analysis of ADA using anti-ADA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ADA antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
ADA / Adenosine Deaminase Antibody - Flow Cytometry analysis of U20S cells using anti-ADA antibody. Overlay histogram showing U20S cells stained with anti-ADA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ADA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - IHC analysis of ADA using anti-ADA antibody. ADA was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml mouse anti-ADA Antibody overnight at 4°C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
ADA / Adenosine Deaminase Antibody - Western blot analysis of ADA using anti-ADA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A549 whole cell lysates, Lane 4: human MCF-7 whole cell lysates, Lane 5: human U-937 whole cell lysates, Lane 6: human U20S whole cell lysates, Lane 7: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-ADA antigen affinity purified monoclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system.
ADA / Adenosine Deaminase Antibody - Flow Cytometry analysis of U20S cells using anti-ADA antibody. Overlay histogram showing U20S cells stained with anti-ADA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ADA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-mouse IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was mouse IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
1 of 7
2 of 7
3 of 7
4 of 7
5 of 7
6 of 7
7 of 7

Monoclonal Mouse anti‑Human ADA / Adenosine Deaminase Antibody (IHC, WB) LS‑C756634

Monoclonal Mouse anti‑Human ADA / Adenosine Deaminase Antibody (IHC, WB) LS‑C756634

Submit Review LSBio Guarantee
Antibody:
ADA / Adenosine Deaminase Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756634-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ADA / Adenosine Deaminase Mouse anti-Human Monoclonal Antibody
Application:
IHC-P, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Adenosine Deaminase antibody LS-C756634 is an unconjugated mouse monoclonal antibody to human Adenosine Deaminase (ADA). Validated for Flow, IHC and WB.
Target
Human ADA / Adenosine Deaminase
Synonyms
ADA | Adenosine deaminase | ADA1 | Adenosine aminohydrolase
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human ADA recombinant protein (Position: Q135-L363). Human ADA shares 82.5% and 82.9% amino acid (aa) sequence identity with mouse and rat ADA, respectively.
Specificity
No cross reactivity with other proteins.
Applications
  • IHC - Paraffin (0.5 - 1 µg/ml)
  • Western blot (0.1 - 0.5 µg/ml)
  • Flow Cytometry (1 - 3 µg/10E6 cells)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADA / Adenosine Deaminase
P00813 NM_000022 NP_000013.2

Publications (0)

Submit a Publication for LS-C756634

Customer Reviews (0)

Submit a review for LS-C756634

Featured Products

ADA / Adenosine Deaminase Antibody - Anti-ADA / Adenosine Deaminase antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.
IHC‑plus™ ADA / Adenosine Deaminase Antibody (aa335‑353) LS‑B792
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
ADA / Adenosine Deaminase Antibody - Immunohistochemistry of paraffin-embedded human cervical cancer using antibody at 1:100 dilution. This image was taken for the unconjugated form of this product. Other forms have not been tested.
ADA / Adenosine Deaminase Antibody (aa157‑343, Biotin) LS‑C369171
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
Product
ADA / Adenosine Deaminase Antibody LS‑C3557
Species: Bovine, Human, Mouse, Rat
Applications: Western blot, ELISA
ADA / Adenosine Deaminase Antibody - ADA Antibody western blot of HL-60 cell line lysates (35 ug/lane). The ADA antibody detected the ADA protein (arrow).
ADA / Adenosine Deaminase Antibody (aa287‑314) LS‑C156031
Species: Human
Applications: Western blot, Flow Cytometry
Product
ADA / Adenosine Deaminase Antibody (aa287‑314, APC) LS‑C250824
Species: Human
Applications: Western blot, Flow Cytometry, Fluorophore-Linked Immunosorbent Assay
Product
ADA / Adenosine Deaminase Antibody (aa287‑314, clone 608CT2.1.3, PE) LS‑C250094
Species: Human
Applications: Western blot, ELISA
See all ADA / Adenosine Deaminase Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
Family of Companies
Site Links Home All Antibodies How To Buy All Proteins Distributors All ELISA & Assay Kits Reviews PathPlus™ Antibodies
Follow LSBio View Cart Register or Login
Copyright © 2022 LifeSpan BioSciences, Inc - Privacy Policy
Contact Us

2 Shaker Rd Suites
B001/B101
Shirley, MA 01464

Tel: +1 (206) 374-1102
Fax: +1 (206) 577-4565

To place an order:
Orders@LSBio.com

For questions about orders:
Customer.Support@LSBio.com

For technical assistance:
Technical.Support@LSBio.com