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Antibodies > ADA / Adenosine Deaminase > LS-C662669
 
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Catalog Number Size Price
LS-C662669-10 10 µg $318 
LS-C662669-100 100 µg $470 
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Western blot analysis of ADA using anti-ADA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADA at approximately 45KD. The expected band size for ADA is at 40KD.
ADA / Adenosine Deaminase Antibody - Western blot analysis of ADA using anti-ADA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates,Lane 2: mouse ovary tissue lysates,Lane 3: rat stomach tissue lysates,Lane 4: rat ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADA at approximately 45KD. The expected band size for ADA is at 40KD.
ADA / Adenosine Deaminase Antibody - Western blot - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Immunohistochemistry - Anti-ADA/Adenosine Deaminase Picoband antibody
ADA / Adenosine Deaminase Antibody - Western blot analysis of ADA using anti-ADA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADA at approximately 45KD. The expected band size for ADA is at 40KD.
ADA / Adenosine Deaminase Antibody - Western blot analysis of ADA using anti-ADA antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: mouse testis tissue lysates,Lane 2: mouse ovary tissue lysates,Lane 3: rat stomach tissue lysates,Lane 4: rat ovary tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ADA antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ADA at approximately 45KD. The expected band size for ADA is at 40KD.
ADA / Adenosine Deaminase Antibody - Western blot - Anti-ADA/Adenosine Deaminase Picoband antibody
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Polyclonal Rabbit anti‑Rat ADA / Adenosine Deaminase Antibody (IHC, WB) LS‑C662669

Polyclonal Rabbit anti‑Rat ADA / Adenosine Deaminase Antibody (IHC, WB) LS‑C662669

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Antibody:
ADA / Adenosine Deaminase Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662669-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
ADA / Adenosine Deaminase Rabbit anti-Rat Polyclonal Antibody
Application:
IHC, IHC-P, WB, ELISA
Reactivity:
Rat
Format:
Unconjugated, Unmodified

Specifications

Description
Adenosine Deaminase antibody LS-C662669 is an unconjugated rabbit polyclonal antibody to rat Adenosine Deaminase (ADA). Validated for ELISA, IHC and WB.
Target
Rat ADA / Adenosine Deaminase
Synonyms
ADA | Adenosine deaminase | ADA1 | Adenosine aminohydrolase
Host
Rabbit
Reactivity
Rat (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Modifications
Unmodified
Immunogen
E. coli-derived rat ADA recombinant protein (Position: A2-H241).
Specificity
Detected in brain and liver (at protein level).
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ADA / Adenosine Deaminase
P00813 NM_000022 NP_000013.2

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 4/28/2024
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