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Work with LifeSpan to design a custom immunohistochemistry to address your specific biological question. Outsource the entire localization process without having to worry about finding and characterizing target specific antibodies, sourcing and validating difficult-to-find tissues, and having the ability to interpret the resulting immunostaining in relation to complex human pathologies.

Test your therapeutic antibodies in immunohistochemistry against a broad panel of normal frozen human tissue types in order to determine potential unintended binding. Our non-GLP TCR services are designed on the FDA recommendation outlined in their "Points to Consider in the Manufacture and Testing of Monoclonal Antibody Products for Human Use".

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ABCC6 / MRP6 Antibody (clone M6II‑31, Concentrated) LS‑C134868

Catalog Number Size Price
LS-C134868-1 1 ml $455 
Antibody

ABCC6 / MRP6 Antibody (clone M6II‑31, Concentrated) LS‑C134868

Antibody:
Rat Monoclonal to Human ABCC6 / MRP6
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Concentrated
Price
Catalog Number
$455 
LS-C134868-1
Toll Free North America
866-819-4732

Overview

Antibody:
Rat Monoclonal to Human ABCC6 / MRP6
Application:
IHC, IHC-P, IHC-Fr, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Concentrated

Specifications

Description
MRP6 antibody LS-C134868 is an unconjugated rat monoclonal antibody to human MRP6 (ABCC6 ). Validated for Flow, IHC and WB.
Target
Human ABCC6 / MRP6
Host
Rat
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG2a Monoclonal
Clone
M6II-31
Conjugations
Unconjugated
Purification
Tissue culture supernatant
Modifications
Concentrated
Applications
  • IHC
  • IHC - Paraffin
  • IHC - Frozen
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Specificity
M6II-31 reacts with MRP6, a 190-200 kD transmembrane protein that is related to the multidrug resistance related protein MRP. Mutations in the MRP6 gene are responsible for the connective tissue disorder Pseudoxanthoma elasticum (PXE). M6II-31 was raised against a bacterial fusion protein of human MRP6, containing amino acids 764-964, spanning the putative 12th transmembrane region as well as predicted internal and external regions of the protein. M6II-7 did not cross-react with the human MDR1, MRP1, MRP2, MRP3, MRP4, MRP5 gene products.
Usage
M6II-31 can be used to detect human MRP6 in cells and tissues. Immunocytochemistry: use 1:20-50 dilution on acetone fixed cytospin preparations. For immunohistochemistry: M6II-31 (use 1:20-50) on acetone fixed frozen sections can be followed by incubation with rabbit anti-rat IgG (1:25, Dako) and an APAAP complex (1:50, Dako). M6II-31 can be used on formaldehyde-fixed paraffin-embedded human tissues, after pretreatment with 0.01 M citric acid (pH 6.0) in distilled water at 100 C for 10 minutes. After incubation of M6II-31 (use 1:20) and washing, slides can be incubated with biotinylated rabbit anti-rat IgG (1:100, Jackson, West Grove) and streptavidin conjugated to horseradish peroxidase (1:500, Zymed, San Francisco, CA). Flow cytometry: optimal conditions still to be defined. Western blotting: use 1:20-50 dilution and anti-rat-HRP.
Presentation
Tissue Culture Supernatant, 0.1% Sodium Azide, 0.7% BSA, Serum-free
Storage
Store at 2-8°C.
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABCC6 / MRP6
O95255 NM_001171 NP_001162.4

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Requested From: United States
Date Requested: 8/23/2019
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