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Catalog Number Size Price
LS-C745315-25 25 µl (0.31 mg/ml) $275 
ABCB5 Antibody - Immunohistochemistry of ABCB5 antibody Tissue: human liver Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: ABCB5 1:200 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain
ABCB5 Antibody - Immunohistochemistry of rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH9. Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal.
ABCB5 Antibody - Immunohistochemistry of rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH6. Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal with hematoxylin purple nuclear counterstain.
ABCB5 Antibody - Western blot using the affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight.
ABCB5 Antibody - Immunohistochemistry of ABCB5 antibody Tissue: human liver Fixation: formalin fixed paraffin embedded Antigen retrieval: user optimized Primary antibody: ABCB5 1:200 Secondary antibody: Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT Localization: Moderate to strong cytoplasmic and membranous staining was observed in hepatocytes. Occasional nuclear staining was observed in hepatocytes and sinusoidal cells. Staining: antibody as precipitated red signal with a hematoxylin purple nuclear counterstain
ABCB5 Antibody - Immunohistochemistry of rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH9. Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal.
ABCB5 Antibody - Immunohistochemistry of rabbit anti ABCB5 antibody. Tissue: human breast carcinoma at pH6. Fixation: formalin fixed paraffin embedded. Primary antibody: ABCB5 antibody at 10 µg/mL for 1 h at RT. Secondary antibody: Peroxidase rabbit secondary antibody at 1:10,000 for 45 min at RT. Localization: ABCB5 is cytoplasmic. Staining: ABCB5 as precipitated brown signal with hematoxylin purple nuclear counterstain.
ABCB5 Antibody - Western blot using the affinity purified anti-ABCB5 antibody shows detection of ABCB5 beta in ~12.5 ug of transfected-Hi5 whole cell lysate. No reaction was seen when antibody was pre-incubated with the immunizing peptide (data not shown). A 3-8% Tris-acetate gel was used for separation. The arrowhead corresponds to 117 kDa ABCB5. The membrane was probed with the primary antibody at a 1:10,000 dilution in 5% milk in TBST at 4° C, overnight.
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Polyclonal Rabbit anti‑Human ABCB5 Antibody (Internal, IHC, WB) LS‑C745315

Polyclonal Rabbit anti‑Human ABCB5 Antibody (Internal, IHC, WB) LS‑C745315

Antibody:
ABCB5 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$275
LS-C745315-25
Toll Free North America
866-819-4732
For Research Use Only

Overview

Antibody:
ABCB5 Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, WB, ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
ABCB5 antibody LS-C745315 is an unconjugated rabbit polyclonal antibody to human ABCB5 (Internal). Validated for ELISA, IHC and WB.
Target
Human ABCB5
Synonyms
ABCB5 | ABCB5alpha | ABCB5 P-gp | ABCB5beta | ATP-binding cassette protein | EST422562 | P-glycoprotein ABCB5
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purified
Modifications
Unmodified
Immunogen
ABCB5 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to residues corresponding to an internal region of human ABCB5.
Epitope
Internal
Specificity
This antibody is specific for human ABCB5 protein. A BLAST analysis was used to suggest partial cross-reactivity with ABCB5 from monkey (85% homology), rat (68% homology) and mouse (62% homology) sources.  Cross-reactivity with ABCB5 from other sources has not been determined.
Applications
  • IHC (1:200)
  • Western blot (1:10000)
  • ELISA (1:10000 - 1:15000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
Applications should be user optimized.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store vial at -20°C or below prior to opening. Dilute 1:10 to minimize loss. Store the vial at -20°C or below after dilution. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABCB5
Q2M3G0 NM_178559 NP_848654.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 5/6/2021