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Catalog Number Size Price
LS-C661867-10 10 µg $318 
LS-C661867-100 100 µg $470 
ABCB11 / BSEP Antibody - Immunohistochemistry - Anti-ABCB11/Bsep Picoband Antibody
ABCB11 / BSEP Antibody - Immunohistochemistry - Anti-ABCB11/Bsep Picoband Antibody
ABCB11 / BSEP Antibody - Immunohistochemistry - Anti-ABCB11/Bsep Picoband Antibody
ABCB11 / BSEP Antibody - IF analysis of ABCB11 using anti-ABCB11 antibody ABCB11 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-ABCB11 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ABCB11 / BSEP Antibody - Western blot - Anti-ABCB11/Bsep Picoband Antibody
ABCB11 / BSEP Antibody - Flow Cytometry analysis of A431 cells using anti-ABCB11 antibody. Overlay histogram showing A431 cells stained with anti-ABCB11 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB11 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
ABCB11 / BSEP Antibody - Immunohistochemistry - Anti-ABCB11/Bsep Picoband Antibody
ABCB11 / BSEP Antibody - Immunohistochemistry - Anti-ABCB11/Bsep Picoband Antibody
ABCB11 / BSEP Antibody - Immunohistochemistry - Anti-ABCB11/Bsep Picoband Antibody
ABCB11 / BSEP Antibody - IF analysis of ABCB11 using anti-ABCB11 antibody ABCB11 was detected in paraffin-embedded section of mouse liver tissues. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution ) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/mL rabbit anti-ABCB11 Antibody overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
ABCB11 / BSEP Antibody - Western blot - Anti-ABCB11/Bsep Picoband Antibody
ABCB11 / BSEP Antibody - Flow Cytometry analysis of A431 cells using anti-ABCB11 antibody. Overlay histogram showing A431 cells stained with anti-ABCB11 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-ABCB11 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human ABCB11 / BSEP Antibody (IHC, WB) LS‑C661867

Polyclonal Rabbit anti‑Human ABCB11 / BSEP Antibody (IHC, WB) LS‑C661867

Antibody:
ABCB11 / BSEP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661867-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
ABCB11 / BSEP Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
BSEP antibody LS-C661867 is an unconjugated rabbit polyclonal antibody to human BSEP (ABCB11). Validated for Flow, ICC, IHC and WB.
Target
Human ABCB11 / BSEP
Synonyms
ABCB11 | ABC16 | Bile salt export pump | BSEP | PGY4 | Sister p-glycoprotein | BRIC2 | PFIC-2 | PFIC2 | SPGP
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human ABCB11 (1175-1199 aa KYGDNTKEIPMERVIAAAKQAQLHD), different from the related mouse and rat sequences by three amino acids.
Specificity
Expressed predominantly, if not exclusively in the liver, where it was further localized to the canalicular microvilli and to subcanalicular vesicles of the hepatocytes by in situ.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About ABCB11 / BSEP
O95342 NM_003742 NP_003733.2

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Requested From: United States
Date Requested: 4/27/2024