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Catalog Number Size Price
LS-C68305-100 100 µg $815 
Antibody

5‑Hydroxytryptophan Antibody LS‑C68305

5‑Hydroxytryptophan Antibody LS‑C68305

Antibody:
Rabbit Polyclonal to 5-Hydroxytryptophan
Application:
IHC, ELISA
Format:
Unconjugated, Unmodified
Price
Catalog Number
$815 
LS-C68305-100
100 µg
Toll Free North America
866-819-4732

Overview

Antibody:
Rabbit Polyclonal to 5-Hydroxytryptophan
Application:
IHC, ELISA
Format:
Unconjugated, Unmodified

Specifications

Description
5-Hydroxytryptophan antibody LS-C68305 is an unconjugated rabbit polyclonal antibody to 5-Hydroxytryptophan. Validated for ELISA and IHC.
Target
5-Hydroxytryptophan
Host
Rabbit
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Purified
Modifications
Unmodified
Applications
  • IHC (1:2000 - 1:5000)
  • ELISA
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Immunogen
5 Hydroxytryptophan Glutaraldehyde Carriers.
Specificity
Recognizes rat 5-Hydroxytryptophan. See additional cross-reactivity data.
Usage
Suitable for use in Immunohistochemistry, ELISA. Immunohistochemistry: 1:2000-1:5000. Tested using the free floating PAP technique on rat raphe nuclei. The anti-conjugated 5-hydroxytryptophan antibodies stains well. Additional cross-reactivity data: Using a conjugate 5 hydroxytryptophan-Glutaraldehyde-Protein, antibody specificity was performed with an ELISA test by competition experiments with the following compounds: Compounds Cross-reactivity ratio (a). 5-Hydroxytryptophan-G-BSA1. 5-Methoxytryptophan-G-BSA1:2000. Tryptophan-G-BSA1:50000. 5-Hydroxytryptamine-G-BSA1:50000. Tryptamine-G-BSA1:>50000. 5-Hydroxytryptophan1:>50000. (a): 5-Hydroxytryptophan-G-BSA concentration/unconjugated or conjugated indolealkylamine concentration at half displacement. G = Glutaraldehyde, BSA. PAP procedure: Second antibody: Sections are incubated with 1/100 dilution of goat anti-rabbit in solution C for 3 hours at 20?C or 1 hour at 37?C. Then, they are washed 3 times (10 min) with solution C. PAP: Sections are incubated with 1/1000 dilution of rabbit peroxidase anti-peroxidase complex in solution C for 1 hour at 37?C. Then, they are washed 3 times (10 min) with solution C. Antibody-antigen complexes are revealed using diaminobenzidine (25 mg/100ml) (or other chromagen) dissolved in Tris 0.05 M and filtrated ; 0.05% of H2O2 is added. The sections are incubated for 10 min at 20?C. Reaction is stopped by transferring sections in 5 ml of Tris 0.05 M.
Presentation
PBS, pH 7.2, No preservatives added
Restrictions
For research use only.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 10/22/2019