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Catalog Number	Size	Price
LS-C775485-100	100 µg (1 mg/ml)	$528

4-HNE Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-4-Hydroxy-2-hexenal Monoclonal Antibody at 1:400 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A,C,E,G) - Untreated. (B,D,F,H) - Cells cultured overnight with 50 µM H2O2. (A,B) DAPI (blue) nuclear stain. (C,D) Phalloidin Alexa Fluor 633 F-Actin stain. (E,F) 4-Hydroxy-2-hexenal Antibody. (G,H) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.

4-HNE Antibody - Western Blot analysis of 4-hydroxy-2-hexanal-BSA Conjugate showing detection of 67 kDa 4-hydroxy-2-hexenal protein using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Lane 1: Molecular Weight Ladder (MW). Lane 2: BSA (0.5 µg). Lane 3: 4-hydroxyl nonenal-BSA (0.5 µg). Lane 4: 4-hydroxy nonenal-BSA (2.0 µg). Lane 5: 4-hydroxy-2-hexenal (0.5 µg). Lane 6: 4-hydroxy-2-hexenal (2.0 µg). Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.

4-HNE Antibody - Flow Cytometry analysis using Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody, Clone 6F10. Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-4-hydroxy-2-hexenal Monoclonal Antibody at 1:50 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Isotype Control: Non Specific IgG. Cells were subject to oxidative stress by treating with 250 µM H2O2 for 24 hours.

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Monoclonal Mouse anti‑All species 4‑HNE Antibody (clone 6F10, FITC, IF, WB) LS‑C775485

Name: Anti-4-HNE Antibody | Mouse anti-All species Monoclonal FITC | LSBio
Brand: Lifespan BioSciences
SKU: LS-C775485-100
Price: 528.0000 USD
Availability: InStock

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Antibody:

4-HNE Mouse Monoclonal (FITC) (6F10) Antibody

Application:

IF, WB, Flo, ELISA

Reactivity:

All species

Format:

FITC, Unmodified

$528

LS-C775485-100

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206-374-1102

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

4-HNE Mouse Monoclonal (FITC) (6F10) Antibody

Application:

IF, WB, Flo, ELISA

Reactivity:

All species

Format:

FITC, Unmodified

Specifications

Description

4-HNE antibody LS-C775485 is an FITC-conjugated mouse monoclonal antibody to all species 4-HNE. Validated for ELISA, Flow, IF and WB.

Target

All species 4-HNE

See All 4-HNE Antibodies

Host

Mouse

Reactivity

All species

Clonality

IgG2b Monoclonal

Clone

6F10

Conjugations

FITC

Purification

Protein G purified

Modifications

Unmodified

Immunogen

Synthetic 4-Hydroxy-2-hexenal modified Keyhole Limpet Hemocyanin (KLH).

Specificity

Specific for 4-Hydroxy-2-hexenal (4-HHE) and 4-Hydroxynonenal (4-HNE) modified proteins. Does not detect free 4-Hydroxy-2-hexenal (4-HHE) or 4-Hydroxynonenal (4-HNE). Does not cross-react with Acrolein, Crotonaldehyde, Hexanoyl Lysine, Malondialdehyde, or Methylglyoxal modified proteins.

Applications