LSBio
Superoxide Dismutase (SOD) Assay Kit (Colorimetric)

Superoxide Dismutase (SOD) Assay Kit (Colorimetric)

Assay FormatEnzyme Activity Assay
Format:Kit
See all Superoxide Dismutase (SOD) antibodies →
$480.00each
Catalog Number: LS-K224-100

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Overview

Description
Superoxide dismutases (SOD, EC1.15.1.1) are enzymes that catalyze the dismutation of superoxide into O2 and H2O2. They are an important antioxidant defense in all cells exposed to O2. There are three major families of superoxide dismutase: Cu/Zn, Fe/Mn, and the Ni type. Aberrant SOD activities have been linked to diseases such as amyotrophic lateral sclerosis, perinatal lethality, neural disorders and cancer. This SOD assay provides a convenient colorimetric means for the quantitative determination of SOD enzyme activity in biological samples. In the assay, superoxide (O2-) is provided by xanthine oxidase (XO) catalyzed reaction. O2- reacts with a WST-1 dye to form a colored product. SOD scavenges the O2- thus less O2- is available for the chromogenic reaction. The color intensity (OD440nm) is used to determine the SOD activity in a sample.
Target
Superoxide Dismutase (SOD)
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Enzyme Activity Assay
Compatible Sample Types
Whole Blood, Cells, Tissue Homogenate
Detection Wavelength (Colorimetric)
440 nm

Format

Format
Kit
Kit Components
Assay Buffer, Diluent, SOD Enzyme, XO Enzyme, Xanthine, WST-1

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.