NAD/NADH Assay Kit (Colorimetric)
Assay KitLSBio Guarantee
| Assay Format | Biochemical Assay |
| Format: | Kit |
$597.00each
Catalog Number: LS-K246-100
Catalog Number: LS-K246
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- Description
- Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. This NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient Method to measure NAD, NADH and their ratio.
- Target
- NAD/NADH
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Cell Lysate, Tissue Homogenate
- Detection Wavelength (Colorimetric)
- 565 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer, Enzyme A, Lactate, Enzyme B, MTT Solution, NAD (P)\/NAD (P)H Extraction Buffer, NAD Standard
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document
