LSBio
Phospholipase D (PLD) Assay Kit (Colorimetric/Fluorometric)

Phospholipase D (PLD) Assay Kit (Colorimetric/Fluorometric)

Assay FormatEnzyme Activity Assay
Format:Kit
See all Phospholipase D (PLD) antibodies →
$480.00each
Catalog Number: LS-K190-100

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Overview

Description
Phospholipase D (PLD) catalyses the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and a free headgroup. Abnormalities in PLD expression have been associated with human cancers. This Method provides a simple and high-throughput assay for measuring PLD activity. In this assay, PLD hydrolyzes phosphatidylcholine to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the PLD activity in the sample.
Target
Phospholipase D (PLD)
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric, Fluorescent / Fluorometric
Assay Type
Quantitative
Assay Format
Enzyme Activity Assay
Compatible Sample Types
Other Biological Sample
Detection Wavelength (Colorimetric)
570 nm

Format

Format
Kit
Kit Components
Assay Buffer, Dye Reagent, Enzyme Mix: Dried Substrate, Calibrator

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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