
Pyruvate Assay Kit (Colorimetric/Fluorometric)
| Assay Format | Biochemical Assay |
| Format: | Kit |
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- Description
- Pyruvate is a key intermediate in cellular metabolic pathways. Pyruvate can be converted to carbohydrates via gluconeogenesis, to fatty acids or energy through acetyl-CoA, to the amino acid alanine and to ethanol. Abnormal levels of pyruvate have been linked to liver diseases and metabolic disorders. Simple, direct and automation-ready procedures for measuring pyruvate concentrations find wide applications in research and drug discovery. This pyruvate assay uses a single Working Reagent that combines pyruvate oxidase and hydrogen peroxide determination in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at lambda ex/em = 530/585nm is directly proportional to pyruvate concentration in the sample.
- Target
- Pyruvate
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric, Fluorescent / Fluorometric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Other Biological Sample, Plasma, Saliva, Serum, Tissue Homogenate, Urine, Milk, Tears
- Detection Wavelength (Colorimetric)
- 570 nm
Format
- Format
- Kit
- Kit Components
- Enzyme Mix, Dye Reagent, Standard 25 mM Pyruvate
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document




















