LSBio
Sorbitol Dehydrogenase (SDH) Assay Kit (Colorimetric)

Sorbitol Dehydrogenase (SDH) Assay Kit (Colorimetric)

Assay FormatEnzyme Activity Assay
Format:Kit
See all Sorbitol Dehydrogenase (SDH) antibodies →
$577.00each
Catalog Number: LS-K239-100

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Overview

Description
SORBITOL DEHYDROGENASE (SDH) is an enzyme that catalyzes the interconversion of sorbitol and fructose. Elevated blood serum SDH levels indicate liver damage; thus, SDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. SDH levels are also measured to evaluate diabetic complications such as proliferative diabetic retinopathy. This non-radioactive, colorimetric SDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Target
Sorbitol Dehydrogenase (SDH)
Category
Assay Kits > All Assay Kits
Alternative Names
L-iditol 2-dehydrogenase | SORD | SORD1 | Sorbitol dehydrogenase

Assay

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Enzyme Activity Assay
Compatible Sample Types
Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate, Urine
Detection Wavelength (Colorimetric)
565 nm

Format

Format
Kit
Kit Components
Assay Buffer, Diaphorase, Substrate, Calibrator, NAD\/MTT Solution

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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