Fumarate Assay Kit (Colorimetric)
Assay KitLSBio Guarantee
| Assay Format | Biochemical Assay |
| Format: | Kit |
$526.00each
Catalog Number: LS-K215-100
Catalog Number: LS-K215
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- Description
- FUMARATE , or Fumaric Acid, is one of the key components in TCA cycle and is used by cells to form ATP. Human skin when exposed to sunlight will naturally produce fumaric acid. Fumarate is used as an additive by the food and beverage industries. Fumaric acid esters are also used to treat psoriasis. Increased urinary fumarate may be due to impaired Krebs cycle function, a defect in the enzyme fumarase or mitochondrial function. This fumarate assay kit is based on fumarase catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm is proportional to the fumarate concentration in the sample.
- Target
- Fumarate
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Food Sample, Other Biological Sample, Plasma, Saliva, Serum, Tissue Homogenate, Urine, Milk, Tears
- Detection Wavelength (Colorimetric)
- 565 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer, Enzyme A, NAD\/MTT, Enzyme B, FMR Enzyme, Standard
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document
