D-Lactate Assay Kit (Colorimetric)
Assay KitLSBio Guarantee
| Assay Format | Biochemical Assay |
| Format: | Kit |
$546.00each
Catalog Number: LS-K236-100
Catalog Number: LS-K236
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- Description
- Lactate is generated by lactate dehydrogenase (LDH) under hypoxic or anaerobic conditions. Monitoring lactate levels is, therefore, a good indicator of the balance between tissue oxygen demand and utilization and is useful when studying cellular and animal physiology. D-lactate is produced in only minor quantities in animals and measuring for D-lactate in animal samples is a means to determine the presence of bacterial infection. Simple, direct and automation-ready procedures for measuring lactate concentration are very desirable. This lactate assay kit is based on lactate dehydrogenase catalyzed oxidation of lactate, in which the formed NADH reduces a formazan (MTT) Reagent. The intensity of the product color, measured at 565 nm, is proportionate to the lactate concentration in the sample.
- Target
- D-Lactate
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Cell Culture Supernatant, Plasma, Serum
- Detection Wavelength (Colorimetric)
- 565 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer, NAD Solution, Enzyme A, MTT Solution, Enzyme B, standard 20 mM D-lactate
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document
