LSBio
Phospholipid Assay Kit (Colorimetric/Fluorometric)

Phospholipid Assay Kit (Colorimetric/Fluorometric)

Assay FormatBiochemical Assay
Format:Kit
See all Phospholipid antibodies →
$577.00each
Catalog Number: LS-K185-100

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Overview

Description
Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. This Method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
Target
Phospholipid
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric, Fluorescent / Fluorometric
Assay Type
Quantitative
Assay Format
Biochemical Assay
Compatible Sample Types
Other Biological Sample, Plasma, Saliva, Serum, Tissue Homogenate, Urine, Milk, Tears
Detection Wavelength (Colorimetric)
570 nm

Format

Format
Kit
Kit Components
Assay Buffer, PLD Enzyme, Enz yme Mix: Dried Dye Reagent, standard 2 mM phosphatidylcholine

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.