
Phospholipid Assay Kit (Colorimetric/Fluorometric)
| Assay Format | Biochemical Assay |
| Format: | Kit |
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- Description
- Phospholipids are a class of lipids which constitute a major component of cell membranes and play important roles in signal transduction. Most phospholipids contain one diglyceride, a phosphate group, and one choline. This Method provides a simple, direct and high-throughput assay for measuring choline-containing phospholipids in biological samples. In this assay, phospholipids (such as lecithin, lysolecithin and sphingomyelin) are enzymatically hydrolyzed to choline which is determined using choline oxidase and a H2O2 specific dye. The optical density of the pink colored product at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the phospholipid concentration in the sample.
- Target
- Phospholipid
- Category
- Assay Kits > All Assay Kits
Assay
- Detection Method
- Colorimetric, Fluorescent / Fluorometric
- Assay Type
- Quantitative
- Assay Format
- Biochemical Assay
- Compatible Sample Types
- Other Biological Sample, Plasma, Saliva, Serum, Tissue Homogenate, Urine, Milk, Tears
- Detection Wavelength (Colorimetric)
- 570 nm
Format
- Format
- Kit
- Kit Components
- Assay Buffer, PLD Enzyme, Enz yme Mix: Dried Dye Reagent, standard 2 mM phosphatidylcholine
Storage & Handling
- Storage Conditions
- Store at -20°C.
Documentation
- Documents
- Document




















