LSBio
Coenzyme A (CoA) Assay Kit (Colorimetric/Fluorometric)

Coenzyme A (CoA) Assay Kit (Colorimetric/Fluorometric)

Assay FormatBiochemical Assay
Format:Kit
See all Coenzyme A (CoA) antibodies →
$561.00each
Catalog Number: LS-K182-100

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Overview

Description
Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of CoA's most crucial roles is the carrying and transferring of acyl groups. This Method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2 reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.
Target
Coenzyme A (CoA)
Category
Assay Kits > All Assay Kits

Assay

Detection Method
Colorimetric, Fluorescent / Fluorometric
Assay Type
Quantitative
Assay Format
Biochemical Assay
Compatible Sample Types
Other Biological Sample, Plasma, Saliva, Serum, Tissue Homogenate, Urine, Milk, Tears
Detection Wavelength (Colorimetric)
570 nm

Format

Format
Kit
Kit Components
Assay Buffer, Dye Reagent, Enzyme A: Dried Enzyme B, Substrate, standard ATP

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.