LSBio
Glutamate dehydrogenase (GLDH) Assay Kit (Colorimetric)

Glutamate dehydrogenase (GLDH) Assay Kit (Colorimetric)

Assay FormatEnzyme Activity Assay
Format:Kit
See all Glutamate dehydrogenase (GLDH) antibodies →
$521.00each
Catalog Number: LS-K169-100

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Overview

Description
GLUTAMATE DEHYDROGENASE (GLDH) is an enzyme which catalyzes the interconversion of glutamate and a-ketoglutarate. Elevated blood serum GLDH levels indicate liver damage; thus, GLDH plays an important role in the diagnosis of liver disease, especially in combination with aminotransferases. Transgenic plants expressing microbial GLDHs are improved in tolerance to herbicide, water deficit, and pathogen infections. This non-radioactive, colorimetric GLDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is directly proportional to the enzyme activity.
Target
Glutamate dehydrogenase (GLDH)
Category
Assay Kits > All Assay Kits
Alternative Names
GLUD1 | GDH1 | GDH 1 | Glutamate dehydrogenase 1 | Glutamate dehydrogenase 2 | GDH | GLUD

Assay

Detection Method
Colorimetric
Assay Type
Quantitative
Assay Format
Enzyme Activity Assay
Compatible Sample Types
Cells, Other Biological Sample, Plasma, Serum, Tissue Homogenate
Detection Wavelength (Colorimetric)
565 nm

Format

Format
Kit
Kit Components
Assay Buffer, Diaphorase, NAD Solution, Calibrator, MTT Solution, Substrate 1 M Glutamate

Storage & Handling

Storage Conditions
Store at -20°C.

Documentation

Documents
Document

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