LSBio
Polyclonal Rabbit anti-Aequorea victoria GFP Antibody (FITC, IF, WB)

Western Blot of GFP Antibody Fluorescein Conjugated. Lane 1: GFP. Load: 50 ng per lane. Primary antibody: none. Secondary antibody: Fluorescein Conjugated Anti-GFP at 1:1000 for 60 min at RT.

Polyclonal Rabbit anti-Aequorea victoria GFP Antibody (FITC, IF, WB)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence, Western Blot
Reactivity:Invertebrate (Other)
Format:Lyophilized
See all Aequorea victoria GFP antibodies →
$555.00each
Catalog Number: LS-C154266-100

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Overview

Description
GFP antibody LS-C154266 is an FITC-conjugated rabbit polyclonal antibody to aequorea victoria GFP. Validated for ELISA, Flow, IF and WB.
Application
ELISA, FC, IF, WB
Reactivity
Invrt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
GFP
Recommended Dilution: ELISA
1:20000 - 1:40000
Recommended Dilution: FC
1:2000
Recommended Dilution: IF/ICC
1:500 - 1:2500
Recommended Dilution: WB
1:10000 - 1:25000
UniProt Number
P42212
SwissProt
P42212

Target

Target
Aequorea victoria GFP
Antigen Species
Invertebrate (Other)
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Fluorescein and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Fluorescein Isothiocyanate
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.