LSBio
GFP Antibody (clone 9F9.F9, TR)

Dot Blot results of Mouse Anti-GFP Antibody Texas Red® Conjugated. Dots are GFP at (1) 100ng, (2) 33.3ng, (3) 11.1ng, (4) 3.70ng, (5) 1.23ng. Primary Antibody: none. Secondary Antibody: Mouse Anti-GFP Antibody Texas Red® at 1µg/mL for 1hr at RT.

GFP Antibody (clone 9F9.F9, TR)

Primary AntibodyLSBio Guarantee
Antibody:Mouse Monoclonal
Applications:Dot Blot, Immunofluorescence, Other, Western Blot
Reactivity:Invertebrate (Other)
Format:Lyophilized
See all Aequorea victoria GFP antibodies →
$966.00each
Catalog Number: LS-C154213-1

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Overview

Description
GFP antibody LS-C154213 is a TR-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for DB, FLISA, IF and WB.
Application
DB, IF, Other, WB
Reactivity
Invrt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names
GFP
Recommended Dilution: IF/ICC
1:500 - 1:2500
Recommended Dilution: WB
1:10000
UniProt Number
P42212
SwissProt
P42212

Target

Target
Aequorea victoria GFP
Antigen Species
Invertebrate (Other)
Epitope
Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by Western blot. No reaction is seen against RFP.

Antibody

Host Species
Mouse
Clonality
Monoclonal
Clone
9F9.F9
Isotype
IgG1
Light Chain
Kappa Light Chain

Immunogen

Immunogen
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Texas Red
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Protein A Affinity

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.