Polyclonal Goat anti-Goat Biotin Antibody (FITC, IF)
Catalog Number: LS-C758060-1
1 mg / $322.00
Antibody: Goat Polyclonal
Applications: Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Immunofluorescence
Reactivity: Goat
Format: Liquid
Western Blot of Goat anti-GFP antibody Peroxidase conjugated. Marker: Opal Pre-stained ladder Lane 2: HEK293 lysate Lane 3: HeLa Lysate Lane 4: CHO/K1 Lysate Lane 5: C2C12 Lysate. Lane 6: NIH/3T3 Lysate Lane 7: Mouse Embryonic Fibroblast Lysate Lane 8: E-coli HCP Control Lane 9: 2 Epitope MBP Tag Marker Lysate Lane 10: Recombinant Red Fluorescent Protein Lane 11: Green Fluorescent Protein Lane 12: Glutathione-S-Transferase Protein. Lane 13: Maltose Binding Protein Marker: Opal Pre-stained ladder Load: 10 µg of lysate or 50ng of purified protein per lane. Primary antibody: goat anti-GFP peroxidase conjugated antibody at 1:1,000 overnight at 4C. Secondary antibody: none
Polyclonal Goat anti-Aequorea victoria GFP Antibody (HRP, IHC, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Goat Polyclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Invertebrate (Other) |
| Format: | Lyophilized |
$764.00each
Catalog Number: LS-C154191-1
Catalog Number: LS-C154191
For bulk orders or custom formulations:
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- Description
- GFP antibody LS-C154191 is an HRP-conjugated goat polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Invrt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Polyclonal Antibodies
- Alternative Names
- GFP
- Recommended Dilution: ELISA
- 1:10000 - 1:50000
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:2000 - 1:5000
- UniProt Number
- P42212
- SwissProt
- P42212
Target
- Target
- Aequorea victoria GFP
- Antigen Species
- Invertebrate (Other)
- Epitope
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, anti-Peroxidase and purified and partially purified Green Fluorescent Protein (Aequorea victoria) Serum. No reaction was observed against Human, Mouse and Rat Serum Proteins.
Antibody
- Host Species
- Goat
- Clonality
- Polyclonal
- Isotype
- IgG
Immunogen
- Immunogen
- Recombinant Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Affinity Purified
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
