Human Apolipoprotein H Antibody (IgG) ELISA Kit (Sandwich ELISA)
Catalog Number: LS-F67372-1
1 plate / $591.00
Reactivity: Human
Detection Method Colorimetric
Format: 96-Well Strip Plate
Peroxidase - Western Blot. Western Blot showing detection of Green Fluorescent Protein (GFP). 50 ng of GFP (Lane 1) was run on a 4-20% gel and transferred to 0.45 micron nitrocellulose. After blocking with 1% BSA-TTBS (MB-013, diluted to 1X) 30 min at 20?, Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate Anti-GFP (MOUSE) Monoclonal Antibody Peroxidase Conjugate - 600-303-215 secondary antibody was used at 1:1000 in Blocking Buffer for Fluorescent Western Blot (p/n MB-070) and imaged using the Bio-Rad VersaDoc 4000 MP. Arrow indicates correct 28 kD molecular weight position expected for GFP. This image was taken for the unconjugated form of this product. Other forms have not been tested.
GFP Antibody (clone 9F9.F9, HRP)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot |
| Reactivity: | Invertebrate (Other) |
| Format: | Lyophilized |
$966.00each
Catalog Number: LS-C154210-1
Catalog Number: LS-C154210
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- GFP antibody LS-C154210 is an HRP-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
- Application
- ELISA, IHC, WB
- Reactivity
- Invrt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- GFP
- Recommended Dilution: ELISA
- 1:20000 - 1:80000
- Recommended Dilution: IHC
- 1:500 - 1:2000
- Recommended Dilution: WB
- 1:2000 - 1:5000
- UniProt Number
- P42212
- SwissProt
- P42212
Target
- Target
- Aequorea victoria GFP
- Antigen Species
- Invertebrate (Other)
- Epitope
- Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase and anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 9F9.F9
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Horseradish Peroxidase
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Thimerosal
- Concentration
- 1 mg/mL
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Store lyophilized at 4°C. Once reconstituted, aliquot and store at -20°C. Avoid freeze/thaw cycles. Store undiluted.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
