GFP Antibody Alkaline Phosphatase Conjugated Western Blot. Western Blot of Mouse anti-GFP Antibody Alkaline Phosphatase Conjugated Lane 1: GFP Load: 100 ng per lane Secondary antibody: GFP Antibody Alkaline Phosphatase Conjugated at 1:1000 for 60 min at RT Block: MB-070 for 30 min at RT Predicted/Observed size: 28 kD, 28 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.

GFP Antibody (clone 9F9.F9, AP)

Primary AntibodyLSBio Guarantee

Antibody:	Mouse Monoclonal
Applications:	Enzyme-Linked Immunosorbent Assay, Immunohistochemistry (general), Western Blot
Reactivity:	Invertebrate (Other)
Format:	Liquid

See all Aequorea victoria GFP antibodies â†’

$1120.00each

Catalog Number: LS-C154211-1

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Overview

Description: GFP antibody LS-C154211 is an AP-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IHC and WB.
Application: ELISA, IHC, WB
Reactivity: Invrt
Intended Use: Research Use Only
Guarantees: This antibody carries the LSBio 100% Guarantee. ✓
Category: Antibodies > Primary Antibodies > Monoclonal Antibodies
Alternative Names: GFP
Recommended Dilution: ELISA: 1:2000 - 1:12000
Recommended Dilution: IHC: 1:200 - 1:1000
Recommended Dilution: WB: 1:500 - 1:2500
UniProt Number: P42212
SwissProt: P42212

Target

Target: Aequorea victoria GFP
Antigen Species: Invertebrate (Other)
Epitope: Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum, anti-Alkaline Phosphatase, purified and partially purified Green Fluorescent Protein (Aequorea victoria). Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by both Western blot and ELISA. No reaction is seen against RFP.

Antibody

Host Species: Mouse
Clonality: Monoclonal
Clone: 9F9.F9
Isotype: IgG1
Light Chain: Kappa Light Chain

Immunogen

Immunogen: Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type: Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label: Alkaline Phosphatase
Format: Liquid
Formulation: 10 mg/mL BSA (Protease and Ig free), 0.05 M Tris-Chloride, pH 8.0, 0.001 M MgCl2, 0.0001 M ZnCl2, 0.15 M NaCl, 0.01% Sodium Azide, 50% (v/v) Glycerol
Concentration: 1 mg/mL
Preservative: Yes
Purification Method: Protein A Affinity