LSBio
GFP Antibody (Biotin)

Immuno-Fluorescence of Biotin Mouse anti-GFP antibody. Biotin mouse anti GFP used 1:5000 As referenced in:

GFP Antibody (Biotin)

Primary AntibodyLSBio Guarantee
Antibody:Rabbit Polyclonal
Applications:Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Immunohistochemistry (general), Western Blot
Reactivity:Invertebrate (Other)
Format:Lyophilized
See all Aequorea victoria GFP antibodies →
$555.00each
Catalog Number: LS-C154288-100

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Overview

Description
GFP antibody LS-C154288 is a biotin-conjugated rabbit polyclonal antibody to aequorea victoria GFP. Validated for ELISA, IF, IHC and WB. Cited in 1 publication.
Application
ELISA, IF, IHC, WB
Reactivity
Invrt
Intended Use
Research Use Only
Guarantees
This antibody carries the LSBio 100% Guarantee.
Category
Antibodies > Primary Antibodies > Polyclonal Antibodies
Alternative Names
GFP
Recommended Dilution: ELISA
1:10000 - 1:50000
Recommended Dilution: IF/ICC
1:5000
Recommended Dilution: IHC
1:1000 - 1:5000
Recommended Dilution: WB
1:2000 - 1:10000
UniProt Number
P42212
SwissProt
P42212

Target

Target
Aequorea victoria GFP
Antigen Species
Invertebrate (Other)
Epitope
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Biotin and purified and partially purified Green Fluorescent Protein (Aequorea victoria). No reaction was observed against Human, Mouse and Rat Serum Proteins.

Antibody

Host Species
Rabbit
Clonality
Polyclonal
Isotype
IgG

Immunogen

Immunogen
Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
Immunogen Type
Fusion Protein (e.g., GST, MBP tag)

Format

Conjugate/Label
Biotin
Format
Lyophilized
Formulation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Concentration
1 mg/mL
Preservative
Yes
Purification Method
Affinity Purified

Storage & Handling

Storage Conditions
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
Recommended Storage Buffer
0.02 M Potassium Phosphate|0.15 M NaCl
ProMAT calibrator: A tool for reducing experimental bias in antibody microarrays. Zangar RC, Daly DS, White AM, Servoss SL, Tan RM, Collett JR. Journal of proteome research. 2009;8:3937-43. PubMed: 19618941

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To request an SDS/MSDS form for this product, please contact our Technical Support department at: technical@vectorlabs.com.