Monoclonal Rat anti-Mouse CD90.2 (Mouse) Antibody (clone 30H12, AF647)
Catalog Number: LS-C810655
2 sizes / from $259.00
Antibody: Rat Monoclonal
Applications: Flow Cytometry
Reactivity: Mouse
Format: Liquid
GFP Antibody Fluorescein Conjugated Western B, ot. Western Blot of GFP Antibody Fluorescein Conjugated Lane 1: GFP Load: 50 ng per lane Secondary antibody: Fluorescein Conjugated Mouse Anti-GFP at 1:1000 for 60 min at RT Block: 1% BSA-TTBS for 30 min at RT Predicted/Observed size: 28 kD, 28 kD. This image was taken for the unconjugated form of this product. Other forms have not been tested.
Monoclonal Mouse anti-Aequorea victoria GFP Antibody (clone 9F9.F9, FITC, IF, WB)
Primary AntibodyLSBio Guarantee
| Antibody: | Mouse Monoclonal |
| Applications: | Enzyme-Linked Immunosorbent Assay, Immunofluorescence, Western Blot |
| Reactivity: | Invertebrate (Other) |
| Format: | Lyophilized |
$966.00each
Catalog Number: LS-C154209-1
Catalog Number: LS-C154209
For bulk orders or custom formulations:
Request a QuoteOverview
- Description
- GFP antibody LS-C154209 is an FITC-conjugated mouse monoclonal antibody to aequorea victoria GFP. Validated for ELISA, IF and WB.
- Application
- ELISA, IF, WB
- Reactivity
- Invrt
- Intended Use
- Research Use Only
- Guarantees
- This antibody carries the LSBio 100% Guarantee. ✓
- Category
- Antibodies > Primary Antibodies > Monoclonal Antibodies
- Alternative Names
- GFP
- Recommended Dilution: ELISA
- 1:20000 - 1:40000
- Recommended Dilution: IF/ICC
- 1:500 - 1:2500
- Recommended Dilution: WB
- 1:10000 - 1:25000
- UniProt Number
- P42212
- SwissProt
- P42212
Target
- Target
- Aequorea victoria GFP
- Antigen Species
- Invertebrate (Other)
- Epitope
- Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein and anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by Western blot. No reaction is seen against RFP.
Antibody
- Host Species
- Mouse
- Clonality
- Monoclonal
- Clone
- 9F9.F9
- Isotype
- IgG1
- Light Chain
- Kappa Light Chain
Immunogen
- Immunogen
- Anti-Green Fluorescent Protein (GFP) is produced by immunizing GFP containing fusion protein that corresponds to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.
- Immunogen Type
- Fusion Protein (e.g., GST, MBP tag)
Format
- Conjugate/Label
- Fluorescein Isothiocyanate
- Format
- Lyophilized
- Formulation
- Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
- Preservative
- Yes
- Purification Method
- Protein A Affinity
Storage & Handling
- Storage Conditions
- Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles.
- Recommended Storage Buffer
- 0.02 M Potassium Phosphate|0.15 M NaCl
